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A High Resolution CCD Intravital Fluorescent Microscope for Observation of Coronary Smaller Microvessels in a Beating Heart

Research Project

Project/Area Number 05557043
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionKawasaki Medical School

Principal Investigator

KAJIYA Fumihiko  Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Takeshi  Kawasaki College of Allied Health Professions, Medical Electronics, Assistant Pr, 医用電子技術科, 講師 (30249560)
MOCHIZUKI Seiichi  Kawasaki College of Allied Health Professions, Medical Electronics, Assistant Pr, 医用電子技術科, 講師 (60259596)
GOTO Masami  Kawasaki College of Allied Health Professions, Medical Electronics, Associate Pr, 医用電子技術科, 助教授 (50148699)
OGASAWARA Yasuo  Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (10152365)
TSUJIOKA Katsuhiko  Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (30163801)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1993: ¥12,500,000 (Direct Cost: ¥12,500,000)
KeywordsHigh Resolution CCD microscope / GRIN Lens / Coronary Circulation / Intramyocardial microvessel / Glomerulus / Intra-renal Microvessel / Needle-probe / Renal Microcirculation / ニードルプローブ / 高品位CCD撮像素子 / 高品位テレビ / 輸入輸出動脈 / 生理活性物質
Research Abstract

We developed a needle-probe intravital microscope with CCD (charge coupled device), by which we can observe coronary microvessels (D>50 mum) in a beating heart with and without fluorescent dye (Circ.Res., 72 : 939-946,1993, Circ.Res.75 : 393-399,1994). To analyze smaller pre- and post-capillary microvessels, we developed a new high resolution CCD system, which consists of a needle probe, a camera body, a lens, a light guide and source, a high definition monitor and a high definition videocassette recorder. The camera has a high resolution CCD image sensor with 1940 x 1040 pixels. The needle-probe contains a relay lens (diameter : 4 mm ; length : 140 mm) to increase resolution. Images focused on the CCD through a relay lens are converted to high definition video signals every 33 ms. To examine the accuracy of this system, a micro scale pattern was observed. The spatial resolution was about 2.5 mum for the x200objective. We could clearly observe small pre- and post-capillary microvessels in subepicardium in a beating heart, which was difficult by our preview system. We also apllied our system to visualize renal microcirculation and found that the images of afferent and efferent arterioles were clealy observed by our system. In conclusion, our new high resolution needle-probe CCD intravital microscope is a powerful tool to observe small pre- and post-capillary microvessels (D<50 mum) in a beating heart.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Toyotaka Yada,et al.: "In Vivo observation of subendocardial microvessels of the beating porcine heart using a needl-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fumihiko Kajiya,et al.: "Erdocardial coronary microcirculation of the beating heart" Advances in Experimental Medicine and Biology. 346. 173-180 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuo Ogasawara,et al.: "A high resolusion CCD intravital microscope for observation of coronary smaller microressels(D<50μm) in a beating heart." Circulation. 90. I265 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tokunori Yamamoto,et al.: "Observation of in Vivo human dye excretion in a single nephron vsing a nsedle-probe CCD videomicroscope" Circulation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toyotaka Yada,et al.: "Effect of nitroglycerin on diameter and pulsation amplitude of subendocardial arterides in beating porcine heart" American Journal of Physiology. 267. H1719-H1725 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Osamu Hiramatsu,et al.: "Diameters of suberdocardial arterioles and venules during prodonged diastole in canine left vertricles" Circulation Research. 75. 393-399 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toyotaka Yada, et al.: "In vivo observation of subendocardial microvessels of the beating porcine heart using a needle-probe videomicroscope with a CCD camera." Circulation Research. 72. 939-946 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fumihiko Kajiya, et al/: "Endocardial coronary microcirculation of the beating heart." Advances in Experimental Medicine and Biology. 346. 173-180 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Osamu Hiramatsu et al.: "Diameters of subendocardial arterioles and venules during prolonged diastole in canine left ventricles." Circulation Research. 75. 393-399 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fumihiko Kajiya et al.: "Novel needle-probe CCD video microscope and its application to organ microcirculation." Physics in Medicine & Biology. 39a. 195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toyotaka Yada et al.: "Nitroglycerin dilates larger arterioles with enhancement to amplitude of systolic-diastolic diameter pulsation in subendocardium." Progress in Microcirculation Research. 363-366 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tokunori Yamamoto et al.: "Observation of in vivo human dye excretion in a single nephron using a needle-probe CCD videomicroscope." Circulation. 90I-266 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toyotaka Yada et al.: "Time-dependent effect of nitroglycerin on subendocardial large and small arterioles." Journal of Cardiovascular Diagnosis and Procedures. 12. 143 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toyotaka Yada et al.: "Effects of nitroglycerin on diameter and pulsation amplitude of subendocardial arterioles in beating porcine heart." American Journal of Physiology. 267. H1719-H1725 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Osamu Hiramatsu et al: "Diameters of Subenclocarclial arterioles and rennles cluring prolonged diastole in canine left rentricles" Clrculation Research. 75. 393-399 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 矢田豊隆他: "ニードルプローブを有するCCDビデオマイクロスコープによる冠・腎微小循環動態解析" Medical Imaging Technology. 12. 149-155 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toyotaka Yada et al: "Effects of nitroglycerin on diameter and pulsation amplitude of suberdocardial arterioles in beating porcine heart" American Journal of Physiology. 267. H1719-H1725 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuo Ogasawara et al: "A high resolusion CCD intravital microscope for observation of coronary smaller imcrovessels (D<50μm)in a beating heart" Circulation. 90. I265 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fumihiko Kajiya et al: "Novel needle‐probe CCD video microscope and its application to organ microcirculation" Physics in Medicine&Biology. 39a. 195 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小笠原康夫他: "CCDマイクロスコープによる冠微小循環計測" 病態生理. 13. 709-714 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toyotaka Yada,etal: "In vivo observation of Subendocordial microvessels of the beating porcihe heart using a needle-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 辻岡克彦、他: "心内膜の血管網をみる" 現代医療. 26. 549-552 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 平松修、他: "ニードル型CCD顕微鏡による拡張期延長時の心内膜側微小冠血管の観察" 第32回日本エム・イー学会大会論文集. 1993. 463

    • Related Report
      1993 Annual Research Report
  • [Publications] Katsuhiko Tsujioka,etal: "In-vivo observation of subendocardial arteriole and venule by a needle-probe videorni croscope with a CCD camera in a beating heart" Abstracts of 2nd Eastern Conference on Medical and Biological Engineering. 284 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Osamu Hiramatsu,etal: "Effect of cardiac contraction on the perforated intramural arteriole and venule in the dog" Circulation. 88. 1757 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 矢田豊隆、他: "反応性充血反応時の拍動下心内膜側冠細動脈に及ぼす影響について" 脈管学. 33. 760 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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