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Application of photoexcited TiO2 semicondoctor to the treatment of bladder cancer

Research Project

Project/Area Number 05557071
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionYokohama City University

Principal Investigator

KUBOTA Yoshinobu  Yokohama City Univ., School of Med, Ass.Professor, 医学部, 助教授 (10106312)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Kazuhito  Univ.Tokyo, School of Engineering, Ass.Professor, 工学部, 助教授 (00172859)
FUJISHIMA Akira  Univ.Tokyo, School of Engineering, Professor, 工学部, 教授 (30078307)
HOSAKA Masahiko  Yokohama City Univ., School of Med, Professor, 医学部, 教授 (30106330)
川崎 千尋  横浜市立大学, 医学部, 講師 (20234057)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordsphotoexcited semiconductor / TiO2 / Bladder cancer / 光半導体癌法
Research Abstract

A photoexcited titanium dioxide surface has a strong ability to decompose water into hydrogen and oxygen. We have studied this effect in order to use it to kill cancer cells in vitro and in vivo. A distinct cell killing effect was observed on cultured T-24 human bladder cancer cells treated with titanium dioxide particles and 300-400 nm UV light irradiation. Titanium dioxide plus UV light also dramatically suppressed the tumour growth of T-24 cells that were implanted in nude mice. Cells cultured on the titanium dioxide electrode were also killed under UV irradiation when the electrode was anodically polarised, suggesting that photogenerated holes are involved in the cell killing. The cell killing effect caused by titanium dioxide particles plus UV light irradiation was significantly hampered in the presence of L-cysteine and catalase, scavengers of hydroxyl radicals and hydrogen peroxide respectively. Transmission electron microscopic observations showed the titanium dioxide particles to be distributed on the cell surface and inside the cells. These results suggest that titanium dioxide particles under UV light irradiation produced photogenerated holes on the surface yielding hydroxyl radicals and hydrogen peroxide inside or outside the cells and the cells were then killed by the action of these highly oxidising molecules. The possible application of photoexcited titanium dioxide particles to cancer treatment as a new anti-cancer modality is discussed.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Cai,R.et al: "Photoelectrochemistry of TiO_2 particles : efficient electron transfer from the TiO_2 particles to a redox enzyme" J.Electroanal.Chem.360. 237-245 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubota,Y.et al: "Photokilling of T-24 human bladder cancer cells with titanium oxide." British Journal of Cancer. 70. 1107-1111 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai,H.et al: "Intra cellular Ca^<2+> concentration change of T-24 cell under irradiation in the presence of TiO_2 ultrafine particle" Biochimica et Biophysia Acta. 1201. 259-265 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai,H.et al: "Selective Killing of a single cancerous T-24 cell with TiO_2 semiconducting microelectrode under irradiation" Chemistry letters. 1995. 185-186 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 窪田吉信他: "光励起半導体を用いる癌の新しい光化学療法" 病態生理. 12. 878-883 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤嶋昭他: "がん治療やMRSA殺菌に応用できる光触媒反応" 表面科学. 16. 24-29 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤嶋昭: "1億人の化学-15-光が活躍する" 日本化学会・大日本図書, 229 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Cai, R., Baba, R., Hashimoto, K., Kubota, Y., Fujishima, A.: "Photoelectrochemistry of TiO2 particles : efficient electron transfer from the TiO2 particles to a redox enzyme" J.Electroanal.Chem.360. 237-245 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kubota, Y., Syuin, T., Kawasaki, C., Hosaka, M., Kitamura, H., Cai, R., Sakai, H., Hashimoto, K., Fujishima, A.: "Photokilling of T-24 humann bladder cancer cells with titanium dioxide" Br.J.Cancer. 70. 1107-1111 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai, H., Ito, E., Cai, R., Yoshioka, T., Kubota, Y., Hashimoto, K., Fujishima, A.: "Intracellular Ca^<2+> concentration change of T-24 cell under irradiation in the presence of TiO_2 ultrafine particle" Biochimica et Biophysica Acta. 259-265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai, H., Baba, R., Hashimoto, K., Kubota, Y., Fujishima, A.: "Serective killing of a single cancerous T-24 cell with TiO_2 semiconducting microelectorode under irradiation" Chemistry Letters. 1995. 185-186 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakai,H.et al: "Selective Killing of a single cancerous T-24 cell with TiO2 Semiconducting Microelectrode under Srradiation" Chemistry Letters. 1995. 185-186 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kubota,Y.et al: "Application of photoexcited TiO2 particle to reglonal cancer treatment a new approach" Regional Cancer Treatment. in press (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koiso,K.et al: "The Status of bladde preservily therapeutic strategies in the management of patients with muscle invasive bladder cancer" Int.J.Urol.2(S). 49-57 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kubota.Y.et al: "Photo kiling of T-24 human bladder cancer cells with titanium dioxide" British Journal of Cancer. 70. 1107-1111 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakai,H.et al: "Intracellular Ca^<2+> conceneration changl of T24 cell under irradiation in the pneseace of ef Tio_2 ultrafine particles" Biochimica et Biophysica Acta. 1201. 259-265 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 窪田吉信 他: "光励起半導体を用いる癌の新しい光化学療法" 病態生理. 12. 878-884 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kubota.Y.et al.: "Prohylaczic Oral UFT therapy for superficial bladder cancer" Cance. 71. 1842-1845 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Cai.R.et al.: "Photalhectrochemistry of TiO_2 particles:efficient electron transfer fromthe TiO_2 particles to a redox enzyme" Journal of Electroanalytoal Chemisery. 360. 237-245 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Miyamoto,H.et al.: "Analysis of PS3 gene mutations in prinary human bladder cance" Oncology Rosencl. 5. 245-249 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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