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Development of New Solution for Preservation of the Liver Graft.

Research Project

Project/Area Number 05557076
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 小児外科
Research InstitutionUniversity of Tokyo

Principal Investigator

TSUCHIDA Yoshiaki (1994-1995)  Department of Pediatric Surgery, University of Tokyo Professor of Pediatric Surgery, 医学部(病), 教授 (80010164)

岩中 督 (1993)  東京大学, 医学部(病), 助手 (90193755)

Co-Investigator(Kenkyū-buntansha) IWANAKA Tadashi  Department of Pediatric Surgeryuniversity of Tokyo, Assistant of Pediatric Surge, 医学部(病), 助手 (90193755)
ITO Mitsuhiro  Department of Surgery, Jichi Medical School Assistant of Surgery, 助手
SUGIYAMA Masahiko  Department of Pediatric Surgeryuniversity of Tokyo, Fellow of Pediatric Surgery, 医学部(病), 医員
KAWARASAKI Hideo  Department of Pediatric Surgery, university of Tokyo Lecturer of Pediatric Surge, 医学部(病), 講師 (60115475)
藤井 光春  ルセル森下製薬株式会社, 開発企画部, 次長
田中 潔  自治医科大学, 医学部, 講師 (60155124)
内田 広夫  東京大学, 医学部(病), 医員
土田 嘉昭  東京大学, 医学部(病), 教授 (80010164)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1995: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1994: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsLiver Transplantation / Solution for Preserving the Liver Graft / Electrolyte Components of the Extra-cellular Fluid / Liver Transplantation in Rats / Liver Transplantation in Monkeys / 臓器保存液 / ラット / hydroxyethyl starch
Research Abstract

The University of Wisconsin (UW) Solution has been shown to preserve all intraabdominal organs sucessfully, especially the liver, for 24 to 48 hours, and liver transplantation after extended preservation with this solution is performed at many institutions and hospitals at present. However, UW solution has a complex composition, must be stored in a refrigerator, and is expensive.
We employed the use of inexpensive TOM-2H solution for simple cold storage of the liver (Iwanaka T,Kawarasaki H,Tsuchida Y,et al : Experimental Orthotopic Liver Transplantation Using TOM-2H Solution, in Which the Major Anion is Gluconate. Transplant. Proc.24 : 1602-1604,1992) , but we found the viscosity of TOM-2H solution is very high and the electrolyte components are of the intracellular type. During the year period of 1993 and 1994, we synthesized an improved TOM solution, a modification of TOM-2H solution, which has low viscosity and extracellular type electrolytes components. The results of experimental orthotopic liver transplantation in rats after 24 hours of simple cold storage using this modified TOM solution were exellent compared with the experiments using TOM-2H soluton and as good as those results using UW solution, in terms of wet liver weight after preservation, ATP and pH values after 1 hour preservation, survivals of the recipient rats, histologic changes of the liver, body weight of the recipients, liver function tests of the recipients, etc.
In 1994 and 1995, we also performed the same experiments using 8 sets of monkeys. However, because of some surgical technical problems, we were unable to obtain sufficient number of survivors to draw conclusions.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 岩中督,土田嘉昭,他: "新しい肝保存液(modified TOM液)を用いた24時間単純冷却浸漬保存後のラット肝移植実験" 移植. 28. 627-630 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawarasaki H,Iwanaka T,Tsuchida Y,et al: "Partial Liver Tranaplantation From a Living Doner : Experimental Research and Clinical Experience" Journal of Pediatric Surgery. 29. 518-522 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Iwanaka T,Tsuchida Y,et al :"Experimental Orthotopic Liver Transplantation Using Modified-TOM Solution in Rats." Ishoku. 28. 627-630 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] kawarasaki H,Iwanaka T,Tsuchida Y,et al :"Partial Liver Transplantation From a Living Donor : Experimental Research and Clinical Experience." J.Pediatr. Surg. 29. 518-522 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuchida, Y, et al: "Evaluation of long-term results of Carolis disease: 21 years of ofservation of a family with autosomal deminant inheritance" Hepatogastioenterology. 42. 175-181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuchida, Y, et al: "Antenatal diagnosis of biliary atresia (Type I cyst) at 19 weekes gestation: Differential diagnosis and etiologic implications" Journal of Pediatric Surgery. 30. 697-699 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 土田嘉昭: "肝内胆管の拡張を示す小児肝胆道疾患の病態とその遠隔成績" 日本小児外科学会雑誌. 31. 701-705 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawarasaki, H, et al: "Liver transplantation from living donor" Hepato-Biliary, Pancreatic and Splenic Disease in Children: Medical and Surgical Management. Elsevet(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuchida, Y.: "Malignant liver and ble duct tumors" Hepato-Biliary, Pancreatic and Splenic Disease in Children: Medical and Surgical Manegement. Elsevet(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuchida, Y.: "Congenital choledocbal cyst: Occurrence of primary intrahepatic stones and prevention of carcinogenesis" Journal of Hepatobiliary and Pancreatic Surgery. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Kawarasaki,T.Iwanaka,Y.Tsuchida, et al: "Partial liver transplantation from a living donor:Experimental research and clinical experience" Journal of Pediatric Surgery. 29. 518-522 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 土田嘉昭: "胆道閉鎖病" 小児外科. 26. 1486-1487 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Tsuchida,H.Kawarasaki et al: "Cyseic dilatation of the intrahepatic biliary system in biliary atresea after hepatic potcenterostomy" Journal of Pediatric Surgery. 29. 630-634 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 岩中 督: "新しい肝保存液(modified TOM液)を用いた24時間単純冷却浸漬保存後のラット肝移植成績" 移植. 28. 627-630 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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