| Project/Area Number |
05557104
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IRIMURA Tatsuro The University of Tokyo, Faculty of Pharmaceutical Sciences Professr, 薬学部, 教授 (80092146)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAYAMA Hiroo Yaklut Central Institute for Microbiological Research, 研究員
YAMAMOTO Kazuo The University of Tokyo Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (20174782)
IMAI Yasuyuki The University of Tokyo Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (80160034)
TSUJI Tsutomu The University of Tokyo Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00143503)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1993: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | Mucin / Monoclonal Antibody / MUC1 / Glycosylation / Cellular / Recognition |
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| Research Abstract |
The aims of this proposal is to establish systems to detect various human epithelial mucins and to establish systems to obtain stable transfectant cells expressing mucin core polypeptide cDNA.Specifically, preparation of monoclonal antibodies specific for unique mucins and preparation of expression vectors containing mucin core polypeptide cDNA were conducted. Elucidation of the transcriptional regulation of mucin core polypeptides was also performed.
The following list consists of the findings obtained during the project term : (1) A novel monoclonal antibody MY.1E12 specific for sialylated MUC1 mucin was established. (2) Vectors containing chimera mucins (MUC1/MMGL and MUC2/MMGL) have been prepared. (3) A stable transfectant cell line and clones have been prepared using human renal carcinoma cells. The biological behavior of these cells were investigated. (4) Glycosylation of MUC1 mucins expressed by transfection of K562 human erythroleukemia cells was compared to the glycosylation of endogenous leukocyte mucins. (5) A lectin expressed by a strain of enterobacterium was isolated and shown to bind MUC2 mucin but not MUC1 mucin. (6) A novel lecting that was isolated from this bacterium. (7) An inducible transcriptional cis-acting element for MUC1 mucin gene has been identified. Binding proteins to this element were isolated from nuclear extracts of mammary carcinoma cells. (8) A cDNA corresponding to human macrophage lectin has been cloned and expressed. The carbohydrate binding specificity of this lectin has been demonstrated to bind to Tn and sialyl-Tn antigens.
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