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Establishment of Detection Systems and Expression Systems for Organ-Specific Mucins

Research Project

Project/Area Number 05557104
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

IRIMURA Tatsuro  The University of Tokyo, Faculty of Pharmaceutical Sciences Professr, 薬学部, 教授 (80092146)

Co-Investigator(Kenkyū-buntansha) TAKAYAMA Hiroo  Yaklut Central Institute for Microbiological Research, 研究員
YAMAMOTO Kazuo  The University of Tokyo Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (20174782)
IMAI Yasuyuki  The University of Tokyo Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (80160034)
TSUJI Tsutomu  The University of Tokyo Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00143503)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1993: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsMucin / Monoclonal Antibody / MUC1 / Glycosylation / Cellular / Recognition
Research Abstract

The aims of this proposal is to establish systems to detect various human epithelial mucins and to establish systems to obtain stable transfectant cells expressing mucin core polypeptide cDNA.Specifically, preparation of monoclonal antibodies specific for unique mucins and preparation of expression vectors containing mucin core polypeptide cDNA were conducted. Elucidation of the transcriptional regulation of mucin core polypeptides was also performed.
The following list consists of the findings obtained during the project term : (1) A novel monoclonal antibody MY.1E12 specific for sialylated MUC1 mucin was established. (2) Vectors containing chimera mucins (MUC1/MMGL and MUC2/MMGL) have been prepared. (3) A stable transfectant cell line and clones have been prepared using human renal carcinoma cells. The biological behavior of these cells were investigated. (4) Glycosylation of MUC1 mucins expressed by transfection of K562 human erythroleukemia cells was compared to the glycosylation of endogenous leukocyte mucins. (5) A lectin expressed by a strain of enterobacterium was isolated and shown to bind MUC2 mucin but not MUC1 mucin. (6) A novel lecting that was isolated from this bacterium. (7) An inducible transcriptional cis-acting element for MUC1 mucin gene has been identified. Binding proteins to this element were isolated from nuclear extracts of mammary carcinoma cells. (8) A cDNA corresponding to human macrophage lectin has been cloned and expressed. The carbohydrate binding specificity of this lectin has been demonstrated to bind to Tn and sialyl-Tn antigens.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Kimura, T.: "Calcium dependent Conformation of a Moose Macrophage Calcium-type lectin." The Jarnal of Biological Chemistry. 270. 16056-16062 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imai, Y.: "Restricted expression of galactose/N-acetylgalactosamine-specific macrophage C-type Lectin to connective〜." Immunology. 86. 591-598 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Konami, Y.: "A. High degree of sequence homology in the potative carbonydrate recognition domains 〜." Glycobiology. 5. 663-670 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki, N: "Molecular Cloning and expression of cDNA encoding Human Macrophage C-Type 〜." The Journal of Immurology. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, M.: "A. Novel Moroclonal Autibody Specifix for Malignancy-Associated Sialylated Mucl Mucin Glycoprotein." Japanese Journal of Cancer Research. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki, N., Yamamoto, K., Osawa, T., Irimura, T: "Molecular coloning and expression of cDNA encoding human macrophage C-type lectin : its unique caarbohydrate binding specificity for Tn antige" J.Immunol. 56^*. 128-135 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, T., Imai.Y., Irimura: "Calcium-dependent conformation of a mouse macrophage calcium-type lectin" J.Biol.Chem. 270. 16056-16062 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Konami, Y., Uno, T., Fujii, M., Yamamoto, K., Osawa, T., Irimura, T: "A high degree of sequence homology in the putative carbohydrate recognition domains of pokeweed mitogen and wheat germ agglutinin : poly-N-acetyllactosamine binding lectins from different species" Glycobiology. 5. 663-670 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imai, y., Akimoto, Y., Mizoguchi, S., Kimura, T., Hirano, H., Irimura, T: "Restricted expression of galactose/ N-acetylgalactosamine-specific macrophage C-type lectin to connective tissue and to metastatic lesions in mouse lung" Immunology. 86. 591-598 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, M., Bhavanandan, V.B., Nakamori, s., Irimura, T: "A novel monoclonal antibody specific for malignancy-assosiated sialylated MUC1 mucin glycoprotein" Jpn.J.Can.Res. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, T.: "Calcium dependent Conformation of a Mouse Macrophage Calcium-type Lectin." The Jarnal of Biological Chemistry. 270. 16056-16062 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Imai, Y.: "Restricted expression of galactose/N-acetylgalactosamine- specific macrophage C-type lectin to connective〜." Immunology. 86. 591-598 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Konami, Y.: "A. High degree of sequence homology in the putative carbohydrate recognition domains〜." Glycobiology. 5. 663-670 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki, N.: "Molecular Cloning and expression of cDNA encoding Human Macrophoge C-Type〜." The Journal of Immunology. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamamoto, M.: "A Novel Moroclonal Autibody Specific for Malignancy -Associated Sialylated MUC1 Mucin Glycoprotein." Japanese Journal of Cancer Research. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakamori,S.: "MUCl mucin explession as a marker of progression and metastasis of human colorectal carcinoma." Gastroenterology. 106. 353-361 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shirotani,K.: "Transcriptional regclation of MUC1 mucin gene in colon carcinoma cells by a soluble Cactol Identification of a regulatory element" J.Biol.Chem.269. 15030-15035 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohe,T.: "Expression of sulfated carbohydrate chains detected by monoclonal antibody 91.9H in human gastric cancer tissues." Jpn.J.Cancer Res.85. 400-408 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto.K.: "Interaction of immobilized recombinant mouse C-type macraphage lectinwith glycopeptides and oligosaccharides." Biochemistry. 33. 8159-8166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,K.: "Role of O-linkod carbohydrate chains on leukocyte cell membranes in platelet-induced leukocyte activation." J.Biol.Chem.269. 23290-23295 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Izomi,Y.: "Characterization of human colon carcinoma variant cell selected for sialyl Le^x carbohydrate antigen:liver colonization and adhecion〜." Exp.Cell Res.216. 215-221 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,K.: "Activated platelets induce superoxide anion release by monocytes and neutrophils throogh P-selectin(CD62)" J.Immunol.151. 3267-3273 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawashima,H.: "Purification and characterization of UDP-GlcNAc:Gal β1-4Glc(NAc)β1,3-N-acetylglycosaminyltranferase(Poly-N-acetyllactosamine〜." J.Biol.Chem.268. 27118-27126 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakamori,S.: "MUC1 mucin expression as a maker of progression and metastasis of human colorectal carcinoma." Gastroenterology. (発表予定). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takeuchi,K.: "Intercellular adhesion induced by anti-α3 integrin(VLA-3)antibodies." Exp.Cell.Res.211(発表予定). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Imai,Y.: "Quantitative measurement of carbohydrate binding activity of mouse macrophage lectin." J.Immunol.Meth.(発表予定). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Konami,Y.: "A unique amino acid sequence involved in the putative carbohydrate-binding domein of alegume lectin specific for sialylated carbohydrate〜." J.Biochem.(発表予定). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Konami,Y.: "A unique amino acid sequence involved in the putative carbohydrate-binding domein of alegume lectin specific for sialylated carbohydrate〜." J.Biochem.(発表予定). (1993)Irimura,T.:

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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