• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of Molecular Pharmacological assay for Ca^<2+> blocker acting on human brain

Research Project

Project/Area Number 05557119
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATOH Masamichi  Kyoto Univ., Faculty of Pharm, Sci, Professor, 薬学部, 教授 (80025709)

Co-Investigator(Kenkyū-buntansha) KANEKO Shuji  Kyoto Univ., Faculty of Pharm, Sci, Associate Professor, 薬学部, 助教授 (60177516)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥11,200,000 (Direct Cost: ¥11,200,000)
Keywordshuman brain / Ca channel / Ca blocker / Xenopus laevis / oocytes / cDNA / molecular cloning / beta subunit / Ca^<2+> / 電位依存性 / アフリカツメガエル卵母細胞 / cDNAクローニング
Research Abstract

1.Complementary DNA for beta subunit of voltage dependent Ca^<2+> channel was cloned from human brain cDNA library and the nucleic acid sequence was determined.
2.Using Xenopus oocytes in which Ca^<2+> channel alpha_1 and beta subunits were coexpressed, practical system for measuring Ca^<2+> channel activity was developed.
3.The beta subunit was nessary for functional expression of Ca^<2+> channels in Xenopus oocytes when they were injected with alpha_1 subunit for neuronal Ca^<2+> channels. Regulatory mechanisms of Ca^<2+> channels by GTP-binding proteins was also clarified.
4.When kappa opioid receptors and Ca^<2+> channels were coexpressed in the oocytes, activity of Ca^<2+> channels was negatively medulated by kappa opioid receptors. Its mechanism was clarified.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko S.et al.: "Ca^<2+>channel inhibition of κ opioid veceptors expressed in Xenopus ooctfes" Neun Reort. 5. (1994)2506-2508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko S,et al.: "Ca^<2+> channel inhibition of kappa opioid receptors expressed in Xenopus oocytes" Neuro Report. 5. 2506-2508 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko S.etal.: "Ca^<2+> channel inhibition by K opioid receptors expressed in Xeuopus oocytes" Neuro Report. 5. 2506-2508 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shunya Nakamura: "Potentiation of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid(AMPA)-selective glutamate receptor function by a nootropic drug,idebenone" Biol.Pharm.Bull.17. 70-73 (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi