| Project/Area Number |
05558002
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
家政学
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| Research Institution | Nara Women's University |
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Principal Investigator |
YOKOIGAWA Kumio Nara Women's Univ.Faculty of Human Life and Environment Associate Prof., 生活環境学部, 助教授 (60230637)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Kinji Junior College of Seibo-Jogakuin, 生活科学科, 教授 (20031643)
KAWAI Hiroyasu Nara Women's Univ.Faculty of Human Life and Environment.Prof., 生活環境学部, 教授 (80026525)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | alanine racemase / food hygiene / polymerase chain reaction / bacteria / foods / detection / ハイブリダイゼーション / DNAプローブ / 生菌数測定 |
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| Research Abstract |
To detect bacteria in foods simply and convenietly, we examined the conditions for amplification of a gene fragment of alanine racemase by polymerase chain reaction. Several primers corresponding to the regions of the active site and His168 to Glu174 of alanine racemase were synthesized and used for this study. Specific amplification of the gene fragment (400bp) was achieved when the PCR was performed at 94 C for 20 s for denaturation, at 54 C for 30 s for annealing, and at 72 C for 180 s for extension. the mehod was applicable for amplification of the gene fragment from various bacteria. When foods such as milk and mineral water containing bacteria was used as templates for the PCR,one bacterial cells in the foods was detected by the PCR.This method is useful for detection of bacteria in foods.
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