AUTOMATED MULTI-CHANNEL LUMINOMETER FOR BIOLUMINESCENCE,DESIG AND ITS APPLICATION FOR CIRCADIAN RHYTHMICITY
| Project/Area Number |
05558079
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioorganic chemistry
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAKAMURA Hideshi HOKKAIDO UNIVERSITY,FACULTY OF SCIENCE ASSOCIATE PROFESSOR, 理学部, 助教授 (90217878)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Takao INSTITUTE FOR BASIC BIOLOGY ASSISTANT, 助手 (10124223)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | BIOLUMINESCENCE / CIRCADIAN RHYTHM / DINOFALGELLATE / CYANOBACTERIA / MULTI-CHANNEL LUMINOMETOR / AUTOMATED SYSTEM |
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| Research Abstract |
Automated mutli-channel luminometer was made by using 16 channel housing, photomultiplier, pocket computer, and Macintosh computer and developted the programs for controlling the machine and analyzing the obtained data. This instrument allowed to follow circadian rhythmicity of two different organisms. Bioluminescent dinoflagellate Gonyaulax polyedra is well know to show circadian rhythmicities in several dinstinct biological events including bioluminescence, cell devision and photosynthetic activity. Under constant light conditions, it showed rhythmicity with a period of about 23 h, however, we could not detect the period-shortening effect of creatine by our luminometor. Since there are many factors influence on the rhythmicity, we could not reproduce the reported effects under various conditions (light intensity, monitoring time, temperature etc.) examined. Gene manipulated cyanobacteria (introducing bacteria luciferase) showed bioluminescence. The light intensity is lower than dinoflagellate but exactly showed circadian rhythmicity with several important characters such as temperature compensation.
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Report
(3 results)
Research Products
(18 results)
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[Publications] Kondo, T., Strayr, C.A., Kulkarni, R.D., Taylor, W., Ishiura.M., Golden S.S., and Johnson C.H.: "Circadian rhythms in prokaryotes : luciferase as a reporter of circadian gene expression in cyanobacteria" Proc.Natl Acad.Sci.90. 5672-5676 (1993)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Kondo T., Tsinoremas, N.F., Golden, S.S., Johnson, C.H., Kutsuna, S., and Ishiura, M.: "Circadian clock mutants of cyanobacteria" Science. 266. 1233-1236 (1994)
Description
「研究成果報告書概要(欧文)」より
Related Report
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