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Development of the basic system for the stereological analysis in the brain

Research Project

Project/Area Number 05558093
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOSAKA Toshio  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,DEPARTMENT OF ANATOMY,PROFESSOR, 医学部, 教授 (00126054)

Co-Investigator(Kenkyū-buntansha) AIKA Yusuke  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,DEPARTMENT OF ANATOMY,ASSOCIATE PROFESSOR, 医学部, 講師 (50021415)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥13,300,000 (Direct Cost: ¥13,300,000)
KeywordsQUANTITATIVE ANALYSIS / STEREOLOGY / DISECTOR / IMMUNOCYTOCHEMISTRY / CONFOCAL LIGHT MICROSCOPE / IMAGE ANALYSIS / NEURON / BRAIN / フォトCD / 中枢神経系 / disector / システマティク・サンプリング
Research Abstract

Detailed structural analyzes, especially quantitative analyzes, are essential to understanding the neuronal organization of the central nervous system and its function. In the present research project we have tried 1) to establish the bases for the stereological analyzes, particularly optical disector analysis, in the central nervous system and 2) to establish the methods of image analyzes for evaluating the immunoreaction intensities of individual somata and synaptic terminals in brain samples at the light microscopic level.
For the optical disector analysis, we stained 50 mm thick sections fluorescent immunocytochemically, and examined by means of a confocal laser scanning light microscope (CLSM). The penetration of immunostaining from the section surfaces was carefully evaluated and the conditions of samples which can be used for the optical disector analysis were established. Then the established methods allowed us to analyze various cell groups defined chemically by means of conventional light microscope as well as CLSM.
The methods for evaluating the intensities of immunoreactivities in individual elements such as neuronal somata and synaptic boutons were developed and applied on determining the distributions of two isozymes of GABA synthesizing enzymes, GAD 67 and GAD 65, in hippocampal GABAergic neurons. Images were acquired by TV camera attached to conventional light microscope for the analyzes of somata and by CLSM for the analyzes of synaptic boutons. Relative reaction intensities on individual elements were analyzed by personal computer using an image analysis software NIH Image.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] T.KOSAKA: "Axons and axon terminals of cerebellar Purkinje cells and basket cells have higher levels of parvalbumin immunoreactivity than somata and dendrites:quantitative analysis by immunogold labeling." Experimental Brain Research. 93. 483-491 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] J.Q.REN: "Regional difference in the distribution of parvalbumin-containing neurons immunoreactive for monoclonal antibody HNK-1 in the mouse cerebral cortex." Neuroscience Letters. 166. 221-225 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat olfactory bulb.1.Distribution and structural features in adult rat." Experimental Brain Research. 99. 191-204 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat olfactory bulb.2.Postnatal development." Experimental Brain Research. 99. 205-213 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.AIKA: "Quantitative analysis of GABA-like immunoreactive and parvalbumin-containing neurons in the CA1 region of the rat hippocampus using a stereological method, the disector." Experimental Brain Research. 99. 267-276 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.TOIDA: "Synaptic contacts between mitral/tufted cells and GABAergic neurons containing calcium binding protein parvalbumin in the rat olfactory bulb, with special reference to reciprocal synapses between them." Brain Research. 650. 347-352 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 佐藤昌康: "ブレインサイエンス最前線'94" 講談社, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.KOSAKA,K.KOSAKA,T.NAKAYAMA,W.HUNZIKER and C.W.HEIZMANN: "Axons and axon terminals of cerebellar Purkinje cells and basket cells have higher levels of parvalbumin immunoreactivity than somata and dendrites : quantitative analysis by immunogold labeling" Experimental Brain Research. 93. 483-491 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] J.Q.REN,C.W.HEIZMANN and T.KOSAKA: "Regional difference in the distribution of parvalbumin-containing neurons immunoreactive for monoclonal antibody HNK-1 in the mouse cerebral cortex" Neuroscience Letters. 166. 221-225 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.KOSAKA,C.W.HEIZMANN and T.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat olfactory bulb. 1. Distribution and structural features in adult rat" Experimental Brain Research. 99. 205-213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.KOSAKA,C.W.HEIZMANN and T.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the ratolfactory bulb. 2. Postnatal development" Experimental Brain Research. 99. 205-213 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.AIKA,J.Q.REN,K.KOSAKA and T.KOSAKA: "Quantitative analysis of GABA-like immunoreactive and parvalbumin-containing neurons in the CA1 region of the rat hippocampus using a stereological method, the disector" Experimental Brain Research. 99. 267-276 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.TOIDA,K.KOSAKA,C.W.HEIZMANN and T.KOSAKA: "Synaptic contacts between mitral/tufted cells and GABAergic neurons containing calcium binding protein parvalbumin in the rat olfactory bulb, with special reference to reciprocal synapses between them" Brain Research. 650. 347-352 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.HAMA,T.ARII and T.KOSAKA: "Three-dimensional organization of neuronal and glial processes : High voltage electron microscopy" Microscopy Research and Technique. 29. 357-367 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.FUJISE,W.HUNZIKER,C.W.HEIZMANN and T.KOSAKA: "Distribution of the calcium binding proteins, calbindin D-28k and parvalbumin, in the subicular complex of the adult mouse" Neuroscience Research. 22. 89-107 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] J.Q.REN: "Regional difference in the distribution of parvalbumin-containing neurons immunoreactive for monoclonal antibody HNK-1 in the mouse cerebral cortex." Neuroscience Letters. 166. 221-225 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat olfactory bulb. 1.Distribution and structural features in adult rat." Experimental Brain Research. 99. 191-204 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat oflactory bulb. 2.Postnatal development." Experimental Brain Research. 99. 205-213 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.AIKA: "Quantitative analysis of GABA-like immunoreactive and parvalbumin-containing neurons in the CA1 region of the rat hippocampus using a stereological method, the disector." Experimental Brain Research. 99. 267-276 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.TOIDA: "Synaptic contacts between mitral/tufted cells and GAB Aergic neurons containing calcium binding protein parvalbumin in the rat olfactory bulb, with special reference to reciprocal synapses between them" Brain Research. 650. 347-352 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.HAMA: "Three-dimensional organization of neuronal and glial processes:High voltage electron microscopy." Microscopy Research and Technique. 29. 357-367 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.KOSAKA: "Axons and axon terminals of cerebellar Purkinje cells and basket cells have higher levels of parvalbumin immunoreactivity than somata and dendrites: puantitative analysis by immunogold labeling." Experimental Brain Research. 93. 483-491 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] J.Q.REN: "Regional difference in the distribution of parvalbumin-coating neurons immunoreactive for monoclonal antibody HNK-1 in the mouse cerebral cortex." Neuroscience Letters. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.AIKA: "Quantitative analysis of GABA-like immunoreactive and parvalbumin-containing neurons in the CA1 region of the rat hippocampus using a stereological method,the desector." Experimental Brain Research. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.KOSAKA: "Calcium-binding protein parbalbumin immunoreactive neurons in the rat olfactory bulb.1.Distribution and structural features in adult rat." Experimental Brain Research. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.KOSAKA: "Calcium-binding protein parvalbumin immunoreactive neurons in the rat olfactory bulb.2.Postnatal development." Experimental Brain Research.

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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