| Project/Area Number |
05558098
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Oita Medical University |
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Principal Investigator |
YAMADA Kazuhiro Oita Med.Univ., Physiol., Prof., 医学部, 教授 (20053027)
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| Co-Investigator(Kenkyū-buntansha) |
IRIGUCHI Norio Siemens Asahi Medical Systems Ltd.MD Application Dept., Assistant Manager, 次長(NMR開発)
NISHIMARU Naoko Oita Med.Univ., Physiol., Assistant Prof., 医学部, 助手 (60101086)
HORI Shigeaki Oita Med.Univ., Neurosurgery., Prof., 医学部, 教授 (00004987)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | magnetic resonance spectroscopy / laser doppler flowmetry / regional lactate / regional blood flow / rabbit brain / hypoxia / oxygen delivery / 筋肉 / 脳 / 非侵襲的方法 / 脳の局在機能 / Nアセチルアスパラギン酸 / 低酸素 |
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| Research Abstract |
Simultaneous applications of localized proton magnetic resonance spectroscopy (1H-MRS) and laser doppler flowmetry (LDF) allowed us to observe concurrent changes in regional lactate level and regional blood flow (rBF) in the anesthetized rabbit brain. The voxel (8mm-cubic) for 1H-MRS was located mostly in the thalamic area, and the rBF was recorded in the same area contralaterally. The localization in the 1H-MRS was assessed by using a phantom model and was proved to be practically satisfactory. During hypoxia, lactate increased gradually, while the rBF increased rapidly. Despite the increase in rBF,the oxygen delivery, which was the oxygen content in arterial blood (O2 CT) multiplied by the rBF,decreased. There was a linear correlation between the increase in lactate in last ten minutes during the thirty minutes hypoxia and deficiency in the oxygen delivery during hypoxia (correlation coefficience ; r2=0.68, n=14, P<0.001). In the subsequent recovery period, the rBF decreased slowly, while the O2 CT increased rapidly back to the control level. This rate of rFB recovery after hypoxia was linearly correlated with the increase in lactate (partial correlation coefficience ; r2=0.72, n=14, p<0.001).
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