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DEVELOPMENT OF INTRAVITAL LASER MICROSCOPE FOR THE OBSERVATION OF OXYGEN DISTRIBUTION

Research Project

Project/Area Number 05558105
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

KAMIYA Akira  UNIV OF TOKYO,FAC OF MED,PROF, 医学部(医), 教授 (50014072)

Co-Investigator(Kenkyū-buntansha) SHIO Megumu  NIKON ENGNEERING CO,DIRECTOR, 開発部長
ANDO Joji  UNIV OF TOKYO,FAC OF MED,ASSOC PROF, 医学部(医), 客員助教授 (20159528)
SHIBATA Masahiro  UNIV OF TOKYO,FAC OF MED,ASSIST PROF, 医学部(医), 講師 (60158954)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥16,500,000 (Direct Cost: ¥16,500,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥14,700,000 (Direct Cost: ¥14,700,000)
KeywordsOXYGEN TENSION / VITAL MICROSCOPY / MICROCIRCULATION / LASER MICROSCOPE / PHOSPHORESCENCE LIFE TIME / 組織代謝 / Stern-Volmer方程式 / 骨格筋 / 組織酸素分圧 / stern-Volmer関係 / 燐光寿命 / 酸素プローブ / 蛍光寿命
Research Abstract

Although it has been recognized for over 100 years that one of the principal functions of the microcirculation is to deliver oxygen to tissue, our understanding of the processes involved in the transport of oxygen from blood to cells is incomplete. A major problem is that we do not know how the concentration of oxygen in tissue varies, particularly in the vicinity of blood vessels and around individual cells. In the present study we used newly designed phosphorescence intravital microscopy, which enable to measure oxygen tension (pO2) in small regions of tissue, to examine the gradient of pO2 in the tissue around the arteriole. Experiments were performed in anesthetized rat cremaster muscle. Pd-porphyrin complexes, the oxygen-dependent : phosphorescence dye were used to measure the quenching decay curve in the tissue. The pO2 was calculated from the time constant of the decay curve, following the Stern-Volmer equation : to/t=1+Kq・to・pO2 where to and are the phosphorescence lifetimes in the absence of O2 and in the tissue being measured and Kq is the quenching constant. Obtained arteriolar pO2 values decreased according to increase the number of branching (1st-order : 70mmHg, 2nd : 55mmHg, 3rd : 45mmHg), however venular pO2 values at the different position showed almost same level(30mmHg). Tissue pO2 values showed large gradient between the area closed to arterioles (arteriolar pO2 minus 15-20mmHg) and 100mum apart (5-8mmHg) . It was concluded that present method offers the continuous, non-invasive determina-tion of oxygen distribution in the skeletal muscle on a microscopic length scale.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] M.Shibata, A.Kamiya et al: "A new flourescent microscopy for tomographic observation of microcirculation by using dual slit laser illumination" Microvascular Research. 49. 300-314 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Baba, M.Shibata et al: "Capillary-tissue arrangement in the skeletal muscle optimiged for oxygen transport in all mammals" Microvascular Research. 49. 163-179 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 神谷 瞭: "血流に対する血管内皮細胞と微小循環系の適応" 医用電子と生体工学(BME). 9. 36-49 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kamiya: "Control of the transcapillary permeability" Microcirculation Annual. 10. 31-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kawamura, M.Shibata et al: "A new intravital microscopic system for measurement of tissue oxygen tension distribution in micron order" Physics in Medicine & Biology. 39. 146 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 柴田政廣 神谷 瞭: "レーザ蛍光顕微鏡による実質臓器内の微小循環計測" 日本レーザ医学会誌. 15. 21-26 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Shibata, T.Kawamura, M.Sohirad, and A.Kamiya: "A new fluorescence microscopy for tomographic observation of microcirculation by using dual-beam slit laser illumination." Microvasc.Res.49. 300-314 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Baba, T.Kawamura, M.Shibata, M.Sohirad, and A.Kamiya: "Capillary-tissue arrangement in the skeletal muscle optimized for oxygen transport in all mammals." Microvasc.Res.49. 163-179 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Shibata, S.Ichioka, K.Kosaki, and A.Kamiya: "Effects of blood flow on capillary angiogenesis." Microcirculation annual. 10 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kamiya: "Control of the transcapillary permeability." Microcirculation annual. 9. 36-49 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Kosaki, A.Kamiya, S.Ichioka, M.Shibata, and A.Kawarada: "In-vivo measurement of structural changes in the microcirculation of the rabbit tenuissimus muscle under long-term prazosin treatment." Microcirculation annual. 9. 187-188 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Shibata, M.Sohirad, and A.Kamiya: "Hetrogeneity of the capillary permeability to macromolecules." Microcirculation annual. 8. 175-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kamiya, M.Shibata, and M.Sohirad :"Macromolecular permeability and hydraulic conductivity through large pores across a single venular capillary."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Veins : Their functional role in the circulation.(S.Hirakawa et al. eds.) Springer-Verlag, Tokyo, 23-32 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Shibata,A.Kamiya,et al: "A new flourescent microscopy for tomographic observation of microcirculation by using dual slit laser illumination" Microvascular Research. (発表予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Baba,A.Kamiya,et al: "Capillary-tissue arrangement in the skeletal muscle optimiged for oxygen transport in all mammals" Microvascular Research. (発表予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 神谷 暸: "血流に対する血管内皮細胞と微小循環系の適応" 医用電子と生体工学(BME). (発表予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kamiya: "Control of the transcapillary permeability" Microcirculation Annual. 10. 31-33 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kawamura,A.Kamiya,et al: "A new intravital microscopic system for measurement of tissue oxygen tension distribution in micron order" Physics in Medicine & Biology. 39. 146 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Shibata,A.Kamiya,et al: "レーザ蛍光顕微鏡による実質臓器内の微小循環計測" 日本レーザ医学会誌. 15. 21-26 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kamiya,J.Ando: "Endothelium-Derived Factors and Vascular Functions" Elsvier Science Publishers B.V., 103-112 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kamiya,J.Ando: "Clinical Biomechanics and Related Researches" Splinger-Verlag Tokyo, 255-271 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kamiya,J.Ando: "Vascular endothelial cell functions and biomechanics" Endothelium. 1. 127-130 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Kamiya,et al.: "Optimality analysis of vascular-tissue system for oxygen transport in mammal" J.Theoretical Biol. 162. 229-242 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,高橋,神谷: "蛍光生体顕微鏡による巨大分子の毛細血管透過性計測法" 医用電子と生体工学. 31. 25-30 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,河村,神谷: "スリットレーザー蛍光生体顕微鏡を用いた実質臓器内微小循環の計測" 脈管学. 33. 1005-1009 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 柴田,神谷: "毛細血管における血流" 病理と臨床. 11. 776-783 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Shibata,M.Sohirad,A.Kamiya: "Heterogeneity of the capillary permeability to macromolecules" Microcirculation aannual. 9. 175-176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Kamiya et al.(共著): "Vein:Functional role in the circulation" Springer-Verlag, 144 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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