| Project/Area Number |
05558109
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
HIROSE Shigehisa Tokyo Institute of Technology Faculty of Bioscience and Biotechnology, 生命理工学部, 教授 (10134199)
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| Co-Investigator(Kenkyū-buntansha) |
AKITA Makoto Schering-Plow Corporation, 研究部, 室長
HAGIWARA Hiromi Tokyo Institute of Technolegy Research Center for Experimental Biology Associate, 生物実験センター, 助教授 (90189465)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | Crosslinking / Transglutaminase / Elafin / Glue / Cementoin / バイオコンジュゲート / ハイブリッド / タンパク質の接着 / 内在性接着因子 |
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| Research Abstract |
The newly found transglutaminase substrates, termed cementoin and cementoin-like moieties, were produced in E.coli using recombinant DNA technology as fusion proteins with maltose-binding protein, purified by affinity chromatography on amyrose resin, and characterized. The products were readily cross-linked with a catalytic amount of transglutaminase, indicating that they have indeed appropriate properties to be used as a protein cross-linker or protein covalent glue. Specific antisera were also produced and used for Western blot analysis, immunohistochemical staining, and radioimmunoassay ; the cross-linker proteins were found to be rich in the epitherium of trachea and intestine. Expression of cementoin/elafin was greatly elevated in a psoriasis patient. These results indicate that cementoin and its homologues are important and useful molecules not only in the field of protein engineering but also in understanding basic physiology.
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