| Project/Area Number |
05558113
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
IWASAKI Hiroshi Osaka University ISIR,PROFESSOR, 産業科学研究所, 教授 (00029901)
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| Co-Investigator(Kenkyū-buntansha) |
MITSUMATA Tadayasu MATSUSHITA ELEC.CO.LTD., RESEARCH WORKER, 中央研究所第一研究室, 室長
YOSHINOBU Tatsuo Osaka University ISIR,RESEARCH ASSOC., 産業科学研究所, 助手 (30243265)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1993: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | bio-chemical-sensor / semiconductor / image-sensor / chemical-image / pH-image / laser scanning / colony / nuron / BZ反応 / pHイメージセンサー / レーザー / バイオセンサー / 食品管理 / 生菌計数 / 顕微鏡 / 大腸菌 |
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| Research Abstract |
A novel chemical imaging microscope has been developed with the aid of the present grant. The chemical imaging microscope is based on the principle of the light-addressable potentiometric sensor (LAPS). The sensing surface of a LAPS device in contact with the electrolyte is made of insulating Si _3N_4 film deposited on semiconducting Si substrate. The two-dimensional chemical information in the electrolyte is mapped into the variation of the width of depletion layr in Si substrate. The electrical impedance of the depletion layr is measured as a function of position, in the form of photocurrent induced by scanning-laser-beam that illuminates the rear surface of the Si substrate.
The spatial resolution, sensitivity and measurement speed have been investigated and improved to be 10 micron meter, 0.01pH and 100 pixels/sec, respectively.
The microscope has been verified to have capability of visualization of two-dimensional bio/chemical information in electrolytic specimens ; distribution of pH of microorganism colonies, specific molecules and redox potential.It could be also used for imaging rapidly changing electrical-potential such as active neuron potential. To demonstrate its applicability to faster processes, we measured the chemical waves in Belousov-Zhabotinsky reaction. Propagation of chemical waves was observed as variation of redox potential either at a fixed point or on a line along the propagating direction. Such measurement would be useful for microscopic analysis of reaction-diffusion and pattern-formation phenomena in redox reaction systems.
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