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Development of Biosensor Comprising DNA as Affinity Ligand

Research Project

Project/Area Number 05558114
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MAEDA Mizuo  Kyushu Univ., Fac.Eng.Assoc.Prof, 工学部, 助教授 (10165657)

Co-Investigator(Kenkyū-buntansha) NAKANO Koji  Kyushu Univ., Fac.Eng.Assoc.Prof, 工学部, 助教授 (10180324)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsDNA Sensor / Biosensor / Gene Sensor / Affinity Sensor / Intercalator / Psoralen / Quinacrine / Cyclic Voltammogram / グルーブバインダー / キナクリン / アクリジンオレンジ / ポリタミン
Research Abstract

A molecular recognition function employed in sensing techniques such as biosensors is usually provided by proteins including enzymes and antibodies. On the other hand, DNA is also an important host molecule in biological affinity reactions. We have developed in this study a new modification chemistry for immobilizing double-helical DNA on metal electrode. Some possible application of the electrode for bioaffinity-based sensing has been studied.
DNA double strands were immobilized on a Au electrode via chemisorption, i.e., a co-ordination to Au with a sulfur-containing moiety. Cyclic voltammograms of ferrocyanide/ferricyanide redox couple were taken with the DNA-modified electrode. When adding quinacrine, the well-known antimalarial drug, the peak currents increased with increasing concentration of the drug. The peak current showed almost a linear relationship with the concentration of quinacrine in the range of 10^<-7>-5x10^<-7>M and then saturated beyond the concentration of 8x^<-7>M.The binding of the cationic drug to the immobilized DNA should reduce the electrostatic repulsion between the polyanionic DNA and the anionic redox couple ions, resulting in the enhancement of the current intensity.
In addition, the DNA-modified electrode was found to be useful for"gene sensor". The sensor comprising DNA as a receptive component can be used for not only the measurement of DNA but also the evaluation of the interaction between DNA and a family of DNA-binding drugs.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Mizuo Maeda: "Semi-Synthetic Macromolecular Conjugates for Biomimetic Seusors" ACS Symposium Series. 556. 238-251 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda: "Mg^<2+>-Selective Electrode Comprising Double-Helical DNA as Receptive Entity" Chem. Lett.1994. 1805-1808 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda: "A Psoralen-Containing Vinyl Monomer for Conjugation of Double-Helical DNA with Vinyl Polymers" Bioconjugate Chem.5. 527-531 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 前田 瑞夫: "DNAを用いるバイオセンサの開発" 日本臨床. 51. 2769-2777 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koji Nakano: "Surface Modification of Au Electrode with Double-Helical DNA for Application to DNA-related Bioaffinity Sensor" The Electrochemical Society, Inc. , Proceedings Volume. 93(11). 423-434 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koji Nakano, Shinji Uchida, Mizuo Maeda, Makoto Takagi: ""Surface Mordification of Au Electrode with Double-Stranded DNA for Application to DNA-Related Bioaffinity Sensor."" The Electrochemical Society, Inc., Proceedings Volume. 93 (11). 423-434 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda, Koji Nakano, Makoto Takagi: ""Semi-Synthetic Macromolecular Conjugates for Biomimetic Sensors."" "Diagnostic Biosensor Polymers, " ed by A.M.Usmani and N.Akmal, ACS Symposium Series, American Chemical Society, Washington D.C.Vol.556. 238-251 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda, Koji Nakano, Shinji Uchida, Makoto Takagi: ""Mg^<2+>-Selective Electrode Comprising Double-Helical DNA as Receptive Entity."" Chem, Lett.1994 (10). 1805-1808 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda, Chitoshi Nishimura, Daisuke Umeno, Makoto Takagi: ""A Psoralen-Containing Vinyl Monomer for Copnjugation of Double-Helical DNA with Vinyl Polymers"" Bioconjugate Chem. 5. 527-531 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroaki Taira, Koji Nakano, Mizuo Maeda, Makoto Takagi: ""Synthesis of Long-Chain, Dialkyl Disulfide Having Terminal Dinitrophenyl Group for Electrode Modification and Application to Impedimetric Immunosennsor."" Anal.Sci. 9. 199-206 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda, Chitoshi Nishimura, Akiko Inenaga, Makoto Takagi: ""Modification of DNA with Poly (N-Isopropylacrylamide) for Thermally Induced Affinity Separation."" Reactive Polym. 21. 27-35 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mizuo Maeda: "Semi-Synthetic macromalecular Conjugates for Biomimetic Sensors" ACS Symposium Series. 556. 238-251 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mizuo Maeda: "Mg^<2+>-Selective Electrode Comprising Double-Helical DNA as Receptive Entity" Chem. Lett.1994. 1805-1808 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mizuo Maeda: "A Psoralen-Containing Vinyl Monomer for Conjugation of Double-Helical DNA" Bioconjugate Chem.5. 527-531 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mizuo Maeda: "Semi-Synthetic Macromolecular Conjugates for Biomimetic Sensors" ACS Symposium Series. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 前田 瑞夫: "DNAを用いるバイオセンサの開発" 日本臨床. 51. 2769-2777 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizuo Maeda: "Modification of DNA with pdy(N-isopropy Lacry Lamide)for Thermally Induced Affinity Separation" Reactive Polym.21. 27-35 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koji Nakano: "Surface Modification of Au Electrode with Double-stranded DNA for Application to DNA-related Bioaffinity Sensor" Proceedings Volume of Electrochemical Society,Inc.93(11). 423-434 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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