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Development of contimous cell separation system using polymeric adsorbent with surface micro-structures

Research Project

Project/Area Number 05558118
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionScience University of Tokyo

Principal Investigator

KATAOKA Kazunori  Science University of Tokyo, Professor, 基礎工学部, 教授 (00130245)

Co-Investigator(Kenkyū-buntansha) NITADORI Yoshiaki  Life Science Research Institute, Asahi Chemical Ind., Co, Ltd., ライフサイエンス総合研究所, 副参事
NAGASAKI Yukio  Science University of Tokyo, Assistant Professor, 基礎工学部, 講師 (90198309)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordscell separation / lymphocyte / chromatography / field flow fractionation / polyamine / graft copolymer / electrostatic interaction / multi-phase polymeric materials / 高分子吸着体 / ミクロドメイン構造
Research Abstract

The interaction of rat lymphocyte subpopulations with the surfaces of poly (2-hydroxyethyl methacrylate) -graft-polyamine copolymer (HA) and poly (2-hydroxyethyl methacrylate) -graft-partially quaternized polyamine copolymer (HQA) was evaluated under a controlled shear stress by using a plane-parallel type channel equipped with a polymer-coated bottom wall. The cells introduced into the channel were settled for a prescribed time period, followed by the exposure to a shear stress resulting from the parabolic flow in the channel. When the shear stress exceeded the strength of cell/surface interaction, the cells were able to be detached from the polymer surface. By this way, quantitative evaluation of cell/surface interaction was done in time-dependent manner to clarify the effect of quaternization of polyamine portion in graft copolymer on lymphocyte attachment. Extended settling time (Tsf) caused a progressive increase in the attachment force between lymphocyte and copolymer surface for HA channel, resulting in a considerable decrease in the recovery of both B and T cells from the channel. On the contrary, recovery of T cells from HQA channel showed only a slight decrease with a settling time, allowing to obtain T cell population as effluent in a good yield even under the condition with prolonged settling time. Time-dependent increase in cell/surface interaction observed for HA channel might be due to a progressive contribution of hydrophobic interaction caused by an increase in hydrophobicity of HA copolymer through the deprotonation of ethylenediamine units in polyamine grafts at cellular interface. These results indicated that lymphocyte attachment is able to be controlled by changing the amount and the nature (fixed our unfixed) of charge in polyamine graft copolymers.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] K. Kataoka: "Development of polymeric adsorbents for separation of lymphocyte subpopulations" Clinical Application of Leukocyte Depletion (Proceedings of The Third Hokkaido Symposium on Transfusion Medicine)Blackwell Sci. , Oxford. 130-137 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y. Nagasaki, E. Honzawa, M. Kato, K. Kataoka, T. Tsuruta: "A synthesis of novel polyamine macrocycles with organosilyl groups" Chemistry Letters. 1993(11). 1825-1828 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A. Kikuchi, T. Tsuruta, K. Kataoka: "Effect of environmental parameters and composition of poly (2-hydroxyethylmethacrylate)-graft-polyaine copolymers on the retention of rat lymphocyte subpopulations (B and T cells)" J. Biomater. Sci. , Polym. Ed.5(6). 569-590 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y. Nagasaki, E. Honzawa, M. Kato, K. Kataoka, T. Tsuruta: "Novel stimuli-sensitive telechelic oligomers-pH and temperature sensitivities of poly (sil-amine) oligomers" Macromolecules. 27(17). 4848-4850 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 平光、田中晶子、片岡一則、鶴田禎二、林正男: "ポリアミングラフト共重合体表面における血管内皮細胞の接着、増殖活性の解析" 人工臓器. 23(3). 695-699 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 平光、片岡一則、鶴田禎二、林正男: "ポリアミングラフト共重合体表面における血管内皮細胞の接着、増殖挙動とその血清タンパク質の影響(in press)" 人工臓器.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 片岡一則、菊地明彦: "細胞分離用材料" 共立出版, 125-143 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Kataoka, A.Kikuchi: "Polymeric materials for cell separation" Biomedical Application of Polymeric Materials Eds.T.Tsuruta, T.Hayashi, K.Ishihara, K.Kataoka, Y.Kimura, CRC,Boca Raton. 345-370 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kikuchi, M.Karasawa, T.Tsuruta, K.Kataoka: "Differential affinity of lymphocyte subpopulations toward PHEMA surface derivatized with a small amount of amino groups-Evaluation under the regulated shear stress" J.Colloid Interface Sci.158 1. 10-18 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kikuchi, M.Karasawa, T.Tsuruta, K.Kataoka: "Amino-containing polymers as non-adsorbable surface of platelets" Artificial Heart 4 (Heart Replacement) , eds., T.Akutsu, H.Koyanagi, R.C.Eberhart, M.Hachida, K.Imachi, K.Kataoka, M.Kitamura, K.Minami, S.Nitta, C,Nojiri, V.L.Poirier, P.M.Portner, Y.Taenaka, S.Takatani, R.Yozu, Springer, Tokyo. 29-32 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Kataoka: "Development of polymeric adsorbents for lymphocyte subpopulations" Clinical Application of Leukocyte Depletion, Ed., S.Sekiguchi, Blackwell Sci., Oxford. 130-137 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Nagasaki, E.Honzawa, M.Kato, K.Kataoka, T.Tsuruta: "A Synthesis of novel polyamine macrocycles with organosilyl groups" Chemistry Letters. 1993 (11). 1825-1828 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kikuchi, T.Tsuruta, K,Kataoka: "Effect of environmental parameters and composition of poly (2-hydroxyethyl methacrylate) -graft-polyamine copolymers on the retention of rat lymphocyte subpoputations (B and T cells)" J.Biomater.Sci., Polym.Ed. 5 (6). 569-590 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Taira, A.Tanaka, K.Kataoka, T.Tsuruta, M.Hayashi: "Adhesion and growth behavior of bovine aortic endothelial cells on polyamine graft copolymer" Jap.J.Artif.Organs. 23 (3). 695-699 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Nagasaki, E.Honzawa, M.Kato, K.Kataoka, T.Tsuruta: "Novel stimuli-sensitive telechelic oligomers-pH and temperature sensitivities of poly (sil-amine) oligomers" Macromolecules. 27 (17). 4848-4850 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Kataoka, H.Koyo, T.Tsuruta: "Novel pH-sensitive hydrogels of segmented polyamineureas having repetitive array of polar and apolar units in the main chain" Macromolecules. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Kataoka, H,Taira, T.Tsuruta, M.Hayashi: "Role of adsorbed fibronectin and vitronectin in adhesion and spreading behavior of bovine aortic endothelial cells on polyamine graft copolymer surfaces" Heart Transplantation (Artificial Heart5) , Eds., T.Akutsu, H.Koyanagi, O.H.Tranzier, M.Hachida, K,Imachi, A.Kantrowitz, K.Kataoka, R.I.Kormos, K.Minami, Y.Mitamura, H.Nishida, S.Nitta, C.Nojiri, Y.Taenaka, S.Takatani, Springer, Tokyo. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Taira, K.Kataoka, T.Tsuruta, M.Hayashi: "Adhesion and growth behavior of bovine aortic endothelial cells on polyamine graft copolymer and influence of serum protein" Jpn.Artif.Organs. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Kikuchi,T.Tsuruta,K.Kataoka: "Effefect of environmental parameters and composotion of poly(2-hydroxycthyl methacrylate)-graft-polyamine copolyemrs on the retention of rat lymphocyte subpopulations(B and T cells)" J.Biomater.Sci.,Polym.Ed.5(6). 569-590 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Nagasaki,E.Honzawa,M.Kato,K.Kataoka,T.Tsuruta: "Novel stimuli-sensitive telechelic oligomers-pH and temperature sensitivities of poly(sil-amine)oligomers" Macromolecules. 27(17). 4848-4850 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 平光、田中晶子、片岡一則、鶴田禎二、林正男: "ポリアミングラフト共重合体表面における血管内皮細胞の接着、増殖活性の解析" 人工臓器. 23(3). 695-599 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 平光、片岡一則、鶴田禎二、林正男: "ポリアミングラフト共重合体表面における血管内皮細胞の接着、増殖挙動とその血清タンパク質の影響" 人工臓器. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Kikuchi,T.Tsuruta,K.kataoka: "Effect of composition and graft‐chain morphology of poly(2-hydroxyethyl methacrylate)‐graft‐polyamine copolymers on the retention of rat lymphocyte subpopulations(B and T cells)" J.Biomater.Sci.,Polym.Ed.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Kataoka: "Clinical Application of Leukocyte Depletion" Blackwell Scientific Pub., 239 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 片岡一則: "ニューマテリアルハンドブック" 昭晃堂, 744 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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