| Project/Area Number |
05558119
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki College of Allied Health Professions |
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Principal Investigator |
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Assistant Professor, 医用電子技術科, 講師 (60259596)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Takeshi Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Assi, 医用電子技術科, 講師 (30249560)
GOTO Masami Kawasaki College of Allied Health Professions, Dept.of Medical Engineering, Asso, 医用電子技術科, 助教授 (50148699)
OGASAWARA Yasuo Kawasaki Medical School, Dept.of Medical Engineering, Assistant Professor, 医学部, 講師 (10152365)
TSUJIOKA Katsuhiko Kawasaki Medical School, Dept.of Medical Engineering, Associate Professor, 医学部, 助教授 (30163801)
KAJIYA Fumihiko Kawasaki Medical School, Dept.of Medical Engineering, Professor, 医学部, 教授 (70029114)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Microdialysis / Bioactive Compounds / Percent Recovery / Kidney / Heart / High Performance Liquid Chromatography / 中空糸膜 / ふるい分け係数 / EDRF |
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| Research Abstract |
We measured the hydraulic permeability and separation characteristics of polyethersulfone membranes before and after adsorption and deposition of plasma proteins and then performed quantitative analysis of membrane performance. The hydraulic permeability of a membrane of 100,000 molecular weight cut off (MWCO) declined 20 to 30% after adsorption of bovine serum albumin. The observed sieving coefficient, which is the ratio of filtrate to bulk concentrations, of polydisperse dextran through a 30,000 MWCO membrane decreased up to 50% after albumin adsorption.
According to the above results, the effects of plasma protein adsorption and deposition on membrane separation performance were significant, and it is crucial for quantitative microdialy methodology to make precise evaluation of recovery rate and % recovery before, during and after microdialysis.
In the second year, we attempted to recover bioactive compounds from some organs such as kidney and heart using a 20,000 MWCO polycarbonate microdialysis probe. In our preliminary experiments, % recovery of adenosine was about 15%, and one of angiotensin II was about 7%. We successfully recovered angiotensin II of the order of ppm from a canine kidney. However, some change in membrane performance seemingly due to protein adsorption was observed, and thus, some pretreatment and/or other membrane materials may need to be considered. In another preliminary experiments of a rat heart using a Langendorff perfusion system, the perfusate from the pulmonary artery was collected, and concentrations of adenosine, inosine, and hypoxanthine were measured using a high performance liquid chromatography. We confirmed continuous monitoring of enhanced production of adenosine and its metabolites during ischemia. We are currently attempting to collect a regional myocardial fluid, especially ATP metabolites using a microdialysis technique.
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