| Project/Area Number |
05559001
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Hokkaido University |
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Principal Investigator |
MIZUNO Morimichi Hokkaido Univ., Sch. of Dent., Inst., 歯学部, 助手 (10125354)
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| Co-Investigator(Kenkyū-buntansha) |
TAZAKI Mariko Sapporo Inst., Sangi Co., Researcher, 札幌研究所, 主任研究員
WATANABE Takanori Sapporo Inst., Sangi Co., Chief., 札幌研究所, 室長
SUZUKI Katsunori Hokkaido Univ., Sch. of Med., Inst., 医学部, 助手 (60241329)
SHIGENOBU Keichi Hokkaido Univ., Sch. of Med., Inst., 医学部, 助手 (30250450)
KUBOKI Yoshinori Hokkaido Univ., Sch. of Dent., Prof., 歯学部, 教授 (00014001)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Osteoblasts / Collagen / Cell differentiation / Bone mrrow cells / 骨髄細胞 / コラーゲンマトリックス |
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| Research Abstract |
In this study, we investigated whether the bone marrow cells-collagen matrix complex induced bone in vivo. Bone marrow cells which were harvested from rat femur, differentiated to osteoblasts on collagen matrix. Under the conventional culture condition, bone marrow cells showed fibroblastic-form, and did not express the typical osteoblastic phenotypes. On the contrary, bone marrow cells on collagen matrix showed polygonal-form, and expressed high alkaline phosphatase activity and synthesized osteocalcin which were markers of osteoblastic phenotypes. Next bone marrow cells-collagen matrix complexes were implanted into the subcutaneous region of nude mouse. Until week two after implantation, collagen matrix remained, and connective tissue were formed between them. On week three, bone was formed, and bone marrow was recognized between bone on week four. No cartilage was formed for the duration of experiment. These findings indicate that bone marrow cells -collagen matrix formed bone in a direct bone formation manner. Bone marrow cells differentiated to mature osteoblasts before the implantation is a prerequisite for the bone formation in vivo. Bone marrow cells themselves or preosteoblasts did not induce bone when combined with collagen matrix and was implanted in vivo. In this project we succeeded to induce bone by the bonemarrow cells and collagen matrix. The precise mechanism of bone formation by bone marrow cells and collagen matrix is unclear. Therefore we are going to continue this project and would like to develop a new therapy for cure the bone defect.
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