Energy Transfer and Photo-induced Electron Transfer in Photofunctional Molecule-Copolypeptide Complexes
| Project/Area Number |
05640574
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Physical chemistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
KUSUMOTO Yoshihumi Kagoshima University, College of Liberal Arts ; Professor, 教養部, 教授 (20094138)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Chlorophy11 / beta-Carotene / Copolypeptide / Pigment-Protein complex model / Energy transfer / Electron transfer / Secondary structure / Surfactant / タンパク質複合体モデル / コポリペプチドの二次構造 / 光誘起電子移動 / 共鳴ラマンスペクトル / 円二色性スペクトル |
|---|
| Research Abstract |
Spectroscopic studies of chlorophyll a (Ch1) and/or beta-carotene (beta-Car) /copolypeptide/surfactant systems have been carried out to model the early events in photosynthesis. The copolypeptides were composed of charged amino acid residues (L-Glu, L-Lys or L-Orn) and uncharged aromatic amino acid residues (L-Tyr, L-Trp or L-Phe) at around pH 6.5. We used cetyltrimethylammonium chloride (CTAC) as a cationic surfactant or sodium dodecyl sulfate (SDS) as anionic Surfactant. The following remarkable results were obtained.
1. The surfactant ion bound cooperatively onto the copolypeptide chain to give a micelle-like cluster. Ch1 and/or beta-car were incorporated into such clusters to from Ch1 and/or beta-Car-copolypeptide complexes with the ordered structure (alpha-helix or beta-structure).
2. The efficiencies of energy transfer from the aromatic amino acid residue or beta-Car to Ch1 in the complexes were determined as a function of surfactant concentration. We also determined energy-transfer parameters such as effective Ch1 concentrations and effective donor-acceptor mean distances on the basis of the Forster theoty.
3. Laser flash photolysis studies of the Ch1 copolypeptide complexes revealed that photo-induced electron transfer took place from the aromatic amino acid residue to Ch1. The electron-transfer pathway mechanisms were discussed.
4. Resonance Raman spectra of Ch1 in the complexes strongly suggested that Ch1 interacted with L-Tyr and L-Trp, most possibly through hydrogen bond, but not L-Phe.
|
Report
(3 results)
Research Products
(7 results)
