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Genetic analyzes of seed storage protein gene expression in response to environmental stimuli

Research Project

Project/Area Number 05640722
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionHokkaido University

Principal Investigator

NAITO Satoshi  Faculty of Agriculture, Hokkaido University Prof., 農学部, 教授 (20164105)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsArabidopsis thaliana / Soy bean / Seed storage protein / Transgenic plants / beta-Glucuronidase / Mutation / Ethionine-resistance
Research Abstract

beta-Conglycinin, one of the major seed storage protein of soybean, consists mainly of three subunits, namelyalpha, alpha' and beta.Among these, the beta subunit is unique because its expression is enhanced under sulfate deficiency and repressed in the presence of methionine.Transgenic Arabidopsis thaliana, SNTbeta3, which carries the beta-glucuronidase (GUS) gene fused to the promoter of the betasubunit gene was constructed.
An A.thaliana mutant, mto1-1, accumulates 10- to 40-fold higher concentration of soluble methionine in rosette leaves during the vegetative growth period.
However, once the mutant plants started to flower, the concentration of soluble methionine in rosette leaves become low and the mutant plants accumulate soluble methionine in immature fruits and the apex region.At 40 days after imbibition, the mutant plants accumulated about 5-fold higher concentration of soluble methionine in these sink organs.
An A.thaliana line that is homozygous for both the mto1-1 mutation and the transgene was constructed (SNTbeta3mto).GUS activity in seeds of SNTbeta3mto plants was 1/40 of that of SNTbeta3 seeds.Reciprocal crosses were made between SNTbeta3 and the mto1-1 mutant plants and the GUS activity was measured in the F1 seeds.The results indicated when the mto1-1 mutant plants were the mother, GUS activity in F1 seeds was repressed while there was no apparent difference in the GUS activity in the reverse cross as compared to the control cross in which wildtype plants were used in place of the mto1-1 mutant plants.These results suggest that soluble methionine translocated from the mother plants regulates the beta subunit promoter activity in seeds.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] S.Naito,: "Expression of a soybean(Glycine max〔L.〕Merr.)seed storage protein gene in transgenic Arabidopsis thaliana and its response to nutritional stress and to abscisic acid mutations." Plant Physiology. 104. 497-503 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Inaba,: "Isolation of an Arabidopsis thalianamutant,mto1,that overaccumulates soluble methionine:temporal and spatial patterns of soluble methionine accumulation." Plant Physiology. 104. 881-887 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Y.Hirai,: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidopsis thaliana." Plant Cell Physiology. 35. 927-934 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Naito,: "Maternal effects of mto1 mutation,that causes overaccumulation of soluble methionine,on the expression of a soybean β-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana." Plant Cell Physiology. 35. 1057-1063 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Naito, M.Y.Hirai, M.Chino and Y.Komeda: "Expression of a soybean (Glycine max[L.]Merr.) seed storage protein gene in transgenic Arabidopsis thaliana and its response to nutritional stress and to abscisic acid mutations." Plant Physiol.104. 497-503 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Inaba, T.Fujiwara, H.Hayashi, M.Chino, Y.Komeda and S.Naito: "Isolation of an Arabidopsis thaliana mutant, mto1, that overaccumulates souble methionine : temporal and spatial patterns of soluble methionine accumulatiom." Plant Physiol.104. 881-887 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Y.Hirai, T.Fujiwara, K.Goto, Y.Komeda, M.Chino and S.Naito: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidosis thaliana." Plant Cell Physiol.35. 927-934 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Naito, K.Inaba-Higano, T.Kanno, E.Nambara, T.Fujiwara, M.Chino and Y.Komeda: "Maternal effects of mto1 mutation, that causes overaccumulation of soluble methionine, on the expression of a soybean beta-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana." Plant Cell Physiol.35. 1057-1063 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Naito,: "Expression of a soybean(Glycine max[L.]Merr.)seed storage protein gene in transgenic Arabidopsis thaliana and its response to nutritional stress and to abscisic acid mutations." Plant Physiology. 104. 497-503 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Inaba,: "Isolation of an Arabidopsis thaliana mutant,mto1,that overaccumulates soluble methionine:temporal and spatial patterns of soluble methionine accumulation." Plant Physiology. 104. 881-887 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Y.Hirai,: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidopsis thaliana." Plant Cell Physiology. 35. 927-934 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Naito,: "Maternal effects of mto1 mutation,that causes overaccumulation of soluble methionine,on the expression of a soybean β-conglycinin gene promoter-GUS fusion in transgeinc Arabidopsis thaliana." Plant Cell Physiology. 35. 1057-1063 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Naito,M.Y.Hirai,M.Chino,Y.Komeda: "Expression of a soybean(Glycine max[L.]Merr.)seed storage protein gene in transgenic Arabidopsis thaliana and its response to nutritional stress and to abscisic acid mutations." Plant Physiology. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Inaba,T.Fujiwara,H.Hayashi,M.Chino,Y.Komeda,S.Naito: "Isolation of an Arabidopsis thaliana mutant,mto1,that over-accumulates soluble methionine:temporal and spatial patterns of soluble methionine accumulation." Plant Physiology. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Yokota Hirai,T.Fujiwara,Y.Komeda,M.Chino and S.Naito: "“Plant Nutrition-from genetic engineering to field practice."" Kluwer Academic Publishers., 803 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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