| Project/Area Number |
05640731
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理
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| Research Institution | Okayama University |
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Principal Investigator |
NAKASHIMA Hideaki Okayama University Faculty of Science Professor, 理学部, 教授 (70142007)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | circadian rhythm / biological clock / Neurospora / calmodulin / サーカディアン |
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| Research Abstract |
The process of light-induced phase shifting was investigated in Neurospora crassa using a liquid culture system and a combination of treatment with a nucleoside analogue and light. 5-Azacytidine inhibited the light-induced phase shifting at all phases that were sensitive to light. Electrophoresis of proteins that were synthesized in a translation system in vitro showed that 5-azacytidine inhibited the synthesis of most mRNAs.the inhibition of mRNA synthesis was correlated with the inhibition of light-induced phase shifting. Newly synthesized mRNA (s) is required during the process of signal tranduction, from the light-perceiving system to the circadian clock, for light-induced phase shifting in Neurospora.
A newly isolated mutant strain of Neurospora crassa, was hypersensitive to chlorpromazine with respect to mycelial growth but was resistant to the drug with respect to the circadian conidiation rhythm.
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