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Light-induced phase-shifting of circadian conidiation rhythm in Neurospora crassa.

Research Project

Project/Area Number 05640731
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionOkayama University

Principal Investigator

NAKASHIMA Hideaki  Okayama University Faculty of Science Professor, 理学部, 教授 (70142007)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordscircadian rhythm / biological clock / Neurospora / calmodulin / サーカディアン
Research Abstract

The process of light-induced phase shifting was investigated in Neurospora crassa using a liquid culture system and a combination of treatment with a nucleoside analogue and light. 5-Azacytidine inhibited the light-induced phase shifting at all phases that were sensitive to light. Electrophoresis of proteins that were synthesized in a translation system in vitro showed that 5-azacytidine inhibited the synthesis of most mRNAs.the inhibition of mRNA synthesis was correlated with the inhibition of light-induced phase shifting. Newly synthesized mRNA (s) is required during the process of signal tranduction, from the light-perceiving system to the circadian clock, for light-induced phase shifting in Neurospora.
A newly isolated mutant strain of Neurospora crassa, was hypersensitive to chlorpromazine with respect to mycelial growth but was resistant to the drug with respect to the circadian conidiation rhythm.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fujimoto,T.,Ohnishi,T.& Nakashima,H: "Ncwly iso lated mutants of Neurospora crassa that can grow optimally in a low-calcium medium" Plant & Cell Physiology. 34. 209-213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Gotou,R.Kaue R,Morishita Y & Nakashima H: "Eltects of temperature on the circadian conidiation rhythm of temperature-sensifive mufants of Nearospora crassa." Plant & Cell Physiology. 35. 613-618 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakashima,H: "Circadian rhythms in plants:Strategy for analysis." J.Plant Research. 106. 283-288 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sadakane.Y & Nakashima.H: "Synthesis of mRNA is reguired for lisht-induced phaseshifting of circadian conidiation rhythm in Neurospora crassa" Plant & Cell Physiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujimoto T,Ohnishi T.and Nakashima H.: "Newly isolated mutants of Neurospora crassa that can grow optimally in a lowcalcium medium." Plant & Cell Physiol.34. 209-213 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Gotou, R., Kaue, R., Morishita, Y.and Nakashima, H.: "Effects of temperature on the circadian conidiation rhythm of temperaturesensitive mutants of Neurosprora crassa." Plant & Cell Physiol.35. 613-618 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakashima, H.: "Circadian rhythms in plants : strategies for analysis." J.Plant Research.106. 283-288 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sadakane, Y.and Nakashima, H.: "Synthesis of mRNA is required for light-induced phase shifting of circadian conidiation rhythm in Neurospora crassa." Plant & Cell Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujimoto.T.Ohnishi T & Nakashima.H.: "Newly isolated mutants of Neurospora crassq that can grow optimally in a low-calcium medium" Plant & Cell Physiology. 34. 209-213 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Gotou.R.Kove.R.Morishita Y and Nakashima.H: "Effects of temperature an the circadian conidiation rhythm of temperature-sensitive mutants of Neurospora crossa." Piant & Cell Physiology. 35. 613-618 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakashima.H: "Circadian rhythms in plants:strategy for analysis" J.Plant Research. 106. 283-288 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sadakane Y and Nakashima H.: "Synthesis of mRNA is required for light induced phase shifting of circadian conidiation rhythm in Neurospora crassa" Plant & Cell Physiol.,. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujimoto,T.: "Newly isolated mutants of Neurospora crassa that can grow optimally in a low-calcium medium." Plant & Cell Physiology. 34. 209-213 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakashima,H.: "Circadian rhythms in plants:strategies for analysis." J.Plant Research. 106. 283-288 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sadakane,Y.: "A requirement for mRNA synthesis in the light-induced phaseshifting of the conidiation rhythm in Neurospora crassa." In“Evolution of circadian clock". (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Gotou,R.: "Effects of temperature on the circadian conidiation rhythm of temperature-sensitive mutants of Neurosprora crassa." Plant & Cell Physiology. (印刷中).

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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