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Mode of steroid action involving in the behavioral regulation

Research Project

Project/Area Number 05640749
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物形態・構造
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TSUTSUI Kazuyoshi  Faculty of Integrated Arts and Sciences, Hiroshima University Associate Professor, 総合科学部, 助教授 (20163842)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsbird / instinct behavior / memory ; learning / brain / neuron / steroid hormone / neurosteroid / neuropeptide / 生殖行動 / ステロイド / ペプチド
Research Abstract

Avian reproductive behaviors are under androgen regulation.To identify the neuropetides that are implicated in the regulation of steroid hormone dependent behavioral processes in birds.We have isolated three opioid peptides, i.e.Met-and Leu-enkephalins and Met-enkephalin-Arg-Phe.This is the first direct demonstration of the presence of opioid peptides in the avian brain.All of the three opioid peptides inhibited the spontaneous firing activities of subsets of neurons in the preoptic and hypothalamic areas that are known to be involved in the regulation of several reproductive behavior.Such an effect was completely blocked by naloxone, an opioid receptor antagonist.It may be possible that opioid peptides regulate some reproductive behavior through the mechanism that provokes the inhibition of neuronal activities.
It is well known that the brain is a target site of steroid hormones supplied by peripheral steroidogenic glands.However, present biochemical experiments demonstrated that the avian brain produces pregnenolone from cholesterol.Most accumulation of pregnenolone in the brain may be independent of the peripheral endocrine gland system.To understand the localization of cells involving with pregnenolone synthesis, the immunohistochemical study with the antibody against cytochrome P450_<scc> was further undertaken in the avian brain.
Clusters of immunoreactive cell bodies were detected in several telencephalic, diencephalic and mesencephalic regions.An intense immunoreaction was detected in somata of Purkinje cells and in the dendrites extending through the cerebellar molecular layr.Western immunoblot analysis with the P450scc antibody confirmed the presence of a P450scc-like substance in these brain regions.Immunohistochemistry using a specific marker protein of glial cells revealed that both neuronal and glial cells possess a P450scc-like substance in the avian brain.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Tsutsui,K.: "Avian neurosteroids:(I)Pregnenolone biosynthesis in the quail brain." Brain Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Usui,M.: "Avian neurosteroids:(II)Localization of a cytochrome P450-like substance in the quail brain." Brain Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Regulation of gonadotropin receptors and its physiological significance in higher vertebrates. (Review)" Zool.Sci.11. 351-361 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Effects of season and photoperiod on the plasma and intratesticular testosteronelevels in Indian weaver birds inhabiting the subtropical zone." J.Exp,Zool.270. 381-387 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Effect of season and photoperiod on the follicle-stimulating hormone receptors in a subtropical bird." J.Biosci.19. 485-489 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakata,S.: "A construction process of behavioral sciences." PTジャーナル. 28. 549-554 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Advances in behavioral sciences." PTジャーナル. 28. 613-620 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Interdisciplinary studies in behavioral sciences." PTジャーナル. 28. 695-698 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Influence of adrenal glands on endocrine factors involved in the regulation of testicular function." Exp.Clin.Endocrinol.12. 43-52 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawashima,S.: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentrations in Indian weaver bird." Gen.Comp.Endocrinol.92. 250-259 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Environmental Impact on Endocrine System in Vertebrates." Follett,B.K.et al., in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 筒井和義: "脳の機能と生物情報「生物科学の基礎」" 焙風館,吉里勝利編, 30 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 筒井和義: "脳の働きの物質的基礎「現代の物質観」" (共立出版,好村滋洋編)(印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.: "Influence of adrenal glands on endocrine factors involved in the regulation of testicular function." Exp.Clin.Endocrinol.12. 43-52 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawashima, S., Tsutsui, K., Saxna, R.N.and Ishii, S.: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentrations in India weaver birds." Gen.Comp.Endocrinol.92. 250-259 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.and Kawashima, S.: "Regulation of gonadotropin receptors and its physiological significance in higher vertebrates. (Review)." Zool.Sci.11. 351-361 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsustui, K.Saxna, T.and Saxna, R.N.: "Effects of season and photoperiod on the plasma and intratesticular testosterone JeveJsin Indian weaver birds inhibiting the subtropical zone." J.Exp.Zool.270. 381-387 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K., Kawashima, S., Saxna, R.N.and Ishii, S.: "Effect of season and photoperiod on the follicle-stimulating hormone receptors in a subtropical bird." J.Biosci.19. 485-489 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakata, S.and Tsutsui, K.: "A construction process of behavioral sciences." PT Journal. 28. 549-554 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.and Sakata, S.: "Advances in behavioral sciences." PT Journal. 28. 613-620 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K., Usui, M.and Sakata, S.: "Interdisciplinary studies in behavioral sciences." PT Journal. 28. 695-698 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.and Yamazaki, T.: "Avian neurosteroids : (I) Pregnenolone biosynthesis in the quail brain." Brain Res. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Usui, M., Yamazaki, T., Kominami, S.and Tsutsui, K.: "Avian neurosteroids : (II)Localization of a cytochrome P450scc like substance in the quail brain." Brain Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K., Usui, M., Yamazaki, T.and Kominami, S.: "Neurosteroids in the avian brain." In : Environmental Impact on Endocrine System in Vertebrates, (B.K.Follett et al., Eds). (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.: Bioligical information and brain function.In : Basic Life Sciences (K.Yoshizato, Ed), Baifukan, (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui, K.: Physical findimgs on the brain function.In : Trends in Physical Studies (S.Komura, Ed), Kyoritsu shupan, (in press), (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutsui,K.: "Avian neurosteroids:(I)Pregnenolone biosynthesis in the quail brain." Brain Res.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Usui,M.: "Avian neurosteroids:(II)Localization of a cytochrome P450-like substance in the quail brain." Brain Res.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Regulation of gonadotropin receptors and its physiological significance in higher vertebrates.(Review)" Zool.Sci.11. 351-361 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Effects of season and photoperiod on the plasma and intratesticular testosterone levels in Indian weaver birds inhabiting the tropical zone." J.Exp.Zool.270. 381-387 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Effect of season and photoperiod on the follicle-stimulating hormone receptors in a subtropical bird." J.Biosci.19. 485-489 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakata,S.: "A construction process of behavioral sciences." PTジャーナル. 28. 549-554 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Advances in behavioral sciences." PTジャーナル. 28. 613-620 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Interdisciplinary studies in behavioral sciences." PTジャーナル. 28. 695-698 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Influence of adrenal glands on endocrine factors involved in the regulation of testicular function." Exp.Clin.Endocrinol.12. 43-52 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawashima,S.: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentrations in Indian weaver birds" Gen.Comp.Endocrinol.92. 250-259 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsutsui,K.: "Environmental Impact on Endocrine System in Vertebrates." Follett,B.K.et al.(in press), (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 筒井和義: "脳の機能と生物情報「生物科学の基礎」" 培風館,古里勝利編, 30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 筒井和義: "脳の働きの物質的基礎「現代の物質観」" (共立出版,好村滋洋編)(印刷中), (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuyoshi Tsutsui: "Influence of adrenal glands on endocrine factors involved in the regulation of testicular function" Exp.Clin.Endocrinol.12. 43-52 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Seiichiro Kawashima: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma concentrations in Indian weaver birds" Gen.Comp.Endocrinol.92. 250-259 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kazuyoshi Tsutsui: "Annual change and photoperiodism of follicle-stimulating hormone receptors in the subtropical bird" J.Biosciences. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 筒井 和義: "脳の機能と生物情報" 生物科学の基礎(吉里勝利編) 培風館. (出版予定). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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