| Project/Area Number |
05640764
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物生理・代謝
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| Research Institution | Toyama University |
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Principal Investigator |
KURODA Hideyo Toyama University, Faculty of Science Professor, 理学部, 教授 (50064845)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUCH Tohru Toyama Med.& Pharmaceu.Univ.Professor, 和漢薬研究所, 教授 (40025680)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | sea urchin / egg / calcium ion / sperm / inositol 1,4,5-trisphosphate / cGMP / Inositol 1,4,5-Trisphosphate / cGMP |
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| Research Abstract |
RESEACH PLAN
We have shown that the application of soluble sperm extract elicits a Ca^<2+>-transient in fertilized, denuded eggs of sea urchin. In this project, we will try to purify this sperm factor and to elucidate how the factor causes the transient.
RESULTS
A sperm factor was partially purified using column chromatography of cation exchanger-anion exchanger-amido 80. The activity was stable in acid, alkali and protease. But we could not succeed further purification. The activity did not probably depend on a single factor.
The Ca^<2+>-transient by sperm extract was inhibited by heparine or GDP-betaS unlike one during fertilization. Furthermore, sperm extract caused a transient increase in IP_3 mass but not in cGMP mass. These results show that sperm extract caused IICR in fertilized eggs but not stimulate CICR.
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