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Molecular evolutionary studies on the origin of eukaryotes-Analyzes of informational macromolecules.

Research Project

Project/Area Number 05640793
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 系統・分類
Research InstitutionThe Nihon University

Principal Investigator

INOUE Tadashi  The Nihon Univ. Dept. Agric. Veter. Med. Associate Prof., 農獣医学部, 助教授 (90124213)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNosema bombycis / Ribosomal RNA / Elongation factor / elongation factor / 微胞子虫 / rRNA遺伝子 / 分子進化 / 真核生物
Research Abstract

In order to investigate the evolutionary origin of eukaryotes, the sequences of ribosomal RNA genes and elongation factors of some microsporidia including Nosema bombycis which is believed to diverge at early stage of eukaryotes are examined by conventional methods.
The originisms used were two strains of N.bombycis, Vairimorpha sp. M12 and Pleistophora sp.. The target genes were PCR-amplified by using appropriate primers from the total genomic DNA which had been extracted from their spores. The complete sequences of the small subunit ribosomal RNA (SSU rRNA) genes were obtained from the above four samples. The lengths of the SSU rRNAs were shorter than those of ordinary prokaryotes, and the secondary structure models constructed from the sequence lacked several stems which are common for the eukaryotic SSU rRNAs. This fact may indicate that the microsporidian SSU rRNA stayd in the primitive evolutional stage.
Attempt to sequence the elongation factors was failed because of the lack of information for the appropriate primers to amplify the gene.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Kawakami,Y.,Inoue,T.,Uchida,Y.,Hatakeyama,Y.,Iwano,H.and Ishinara,R.: "Specific amplification of DNA from reference strains of Nosema bombycis(Nosematidae,Microsporidia)." J.Seric.Sci.Jpn.64(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawakami, Y., Inoue, T., Uchida, Y., Hatakeyama, Y., Iwano, H.and Ishihara, R.: "Specific amplification of DNA from reference starains of Nosema bombycis (Nosematidae, Microsporidia) ." J.seric.Sci.Jpn.64 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawakami,Y.,Inoue,T.,Uchida,Y.,Hatakeyama,Y.,Iwano,H.and Ishihara,R.: "Specific amplification of DNA from reference strains of Nosema bombycis(Nosematidae,Microsporidia)." J.Seric.Sci.Jpn.64(in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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