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Development of artificial liver using macroporous support

Research Project

Project/Area Number 05650797
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物・生体工学
Research InstitutionNagoya university

Principal Investigator

HONDA Hiroyuki  Nagoya University Faculty of Engineer., Dept.of Biotechnol., Associate Prof., 工学部, 助教授 (70209328)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Takeshi  Nagoya University Faculty of Engineer., Dept.of Biotechnol., Prof., 工学部, 教授 (10043324)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
KeywordsArtificial liver / Rat hepatocyte / three dimensional culture / immobilized support / albumin / glucose / long term culture / mammalian cell culture / コラーゲン / 細胞接着因子
Research Abstract

1. To establish a three dimensional culture of mammalian cell, anchorage dependent or independent cell culture was carried out by using some macroporous cell supports, such as a support linked with heparin or cell adhesion factor and with positive or negative charged groups. In the culture using a cellulose macroporous support, relatively high cell density was obtained. It was found that the highest value of the maximum cell density and the production of biochemicals was obtained in the cellulose support with positive charged group.
2. To construct a bioreactor packed with a artificial liver for clinical use, long term cultivation of rat hepatocyte with hepatocyte specific function should be established. For this purpose, the effect of additives on albumin secretion rate as a hepatocyte specific function was studied. The albumin secretion rate as a specific hepatocyte function was checked in dish culture of rat hepatocyte. Although the hepatocyte function including an albumin secretion rate decrease gradually during cultivation, it was found that 2 to 3 times higher rate was obtained by addition of anti-oxidative agent such as proline and histidine among amino acids, catalase and superoxide dismutase among enzymes, ascorbic phosphate ester and transferrine.
3. The effect of glucose concentration on hepatocyte specific function was studied. Gulucose was added at the variable levels from 0.1 to 3.0 mM in the dish culture. It was found that the secretion rate in the early stage increased with decrease of glucose concentration. When glucose concentration was shifted up from low level (0.1mM) to high level (10 mM) at various culture days by changing the medium, high secretion rate was also obtained. It was found that when the medium was changed after 4 days, 2 times higher rate was kept even through after 14 days. This result means that the glucose level controlled culture become one of the effective strategy for long term hepatocyte culture.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 本多 裕之: "EFFECT OF ADDITIVES SUCH AS ANTI-OXIDATIVE AGENT ON ALBUMIN SECRETION FROM PRIMARY CULTURE OF RAT HEPATOCYTE." Biotechnology Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroyuki Honda: "EFFECT OF ADDITIVES SUCH AS ANTI-OXIDATIVE AGENT ON ALBUMIN SECRETION FROM PRIMARY CULTURE OF RAT HEPATOCSYTE." Biotechnology Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 本多 裕之: "EFFECT OF ADDITIVES SUCH AS ANTI-OXIDATIVE AGENTON ALBUMIN SECRETION FROM PRIMARY CULTURE OF RATHEPATOCYTE.21GC01:Biotechnology Letters" (in press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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