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Optimal designing of animal cell bioreactors by three-dimensional flow simulation using super-computer

Research Project

Project/Area Number 05650804
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物・生体工学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MATSUSHITA Taku  Kyushu Univ.・Dept.Chem.Eng.・Res.Assoc., 工学部, 助手 (10209538)

Co-Investigator(Kenkyū-buntansha) KAJIWARA Toshihisa  Kyushu Univ.・Dept.Chem.Eng.・Assoc.Prof., 工学部, 助教授 (10194747)
FUNATSU Kazumori  Kyushu Univ.・Dept.Chem.Eng.・Prof., 工学部, 教授 (80037960)
中野 祥生  九州大学, 工学部, 助手 (70237353)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsThree-dimensional flow simulation / Super-computer / Animal cell culture / Agitated bioreactor / Shear stress / Shear stress history / Particle tracking method / 293 cells / 攪拌槽型培養装置 / 攪拌槽培養装置 / 細胞付着力 / 血栓溶解酵素
Research Abstract

1. We have developed the simulation technique for 3-dimensional flow of a Newtonian fluid in agitated bioreactors using finite element method and supercomputer. Numerical and experimental results were in good agreement with each other concerning velocity and snear stress distributions. Therefore, the technique was found applicable for flow desing of optimal agitated bioreactors.
2. It was suggested by this research that there are two manner of cell damage caused by shear stress. One is a critical shear stress which causes cell breakage instantly. The other is a shear stress history for long culture period which causes a decrease of cell viability even if at a lower level of shear stress.
3. We have succeeded in estimating a shear stress history in a commercial spinner flask with 2 bladed paddle impellers at the rotational speed of 60 rpm, where the time interval is 0.02 s and the total tracking time is 90 s, by a newly developed particle tracking simulation technique. The particle rotates circularly with gradual going down in the axial direction and then rising upward from the bottom of the flask, and finally almost returns to the initial position. The average of its one cycle time was 30.6 s, and the average of shear stress history per one cycle was 0.134 Pa・s. At next step, it is important to elucidate a relation between the shear stress history and cell damage for long culture time.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kazumori Funatsu: "Finite Element Method Analysis of 3-D Flow in an Agitated Bioreactor" Cell Culture Engineering IV abstract. 117- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Keiichirou Fukushima: "Estimation of a shear stress history of the animal cells in an agitated bioreactor" 化学工学会第27回秋季大会研究発表講演要旨集. 第3分冊. 180- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuhiko Kawakubo: "T-PA production by high density culture of weakly adherent human embryonic kidney cells using polyurethane foam packed-bed culture" Applied Microbiology and Biotechnology. 41. 413-418 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 福島圭一郎: "三次元流動シミュレーションによる撹拌槽型バイオリアクターの最適設計に関する検討" 化学工学会第59年会研究発表講演要旨集. 第2分冊. 32- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Taku Matsushita: "Finite Element Analysis of 3-Dimensional Flow in an Agitated Bioreactor" 化学工学シンボジウムシリーズ. (発表予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: "Finite Element Analysis of 3-D Flow in an Agitated Bioreactor" Cytotechnology. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 松下 琢: "バイオプロセスシステム工学 -第6章 動物細胞培養-" 株式会社アイピーシー, 687 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: "BETTER LIVING THROUGH INNOVATIVE BIOCHEMICAL ENGINEERING" Continental Press Pte Ltd, 880 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: "Finite Element Method Analysis of 3-D Flow in an Agitated Bioreactor" Cell Culture Engineering IV abstract. 117 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Keiichirou Fukushima: "Estimation of a shear stress history of the animal cells in an agitated bioreactor" Proc.of the 27th Autumn Meeting of Soc.Chem.Eng.Jpn. Vol.3. 180 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuhiko Kawakubo: "T-PA production by high density culture of weakly adherent human embryonic kidney cells using polyurethane foam packed-bed culture" Applied Microbiology and Biotechnology. 41. 413-418 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Keiichirou Fukushima: "Studies on optimal designing of an agitated bioreactor by using three-dimensional flow simulation" Proc.of the 59th Annual Meeting of Soc.Chem.Eng.Jpn. Vol.2. 32 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Taku Matsushita: "Finite Element Analysis of 3-Dimensional Flow in an Agitated Bioreactor" Chemical Engineering Symposium Series, -Biochemical Engineering as a Key Technology for Bioindustry-. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: "Finite Element Analysis of 3-D Flow in an Agitated Bioreactor" Cytotechnology. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Taku Matsushita: Bioprocess System Engineering Chapter 6 ; Animal cell culture. I.P.C.Inc.Co., 687 (221-254) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: BETTER LIVING THROUGH INNOVATIVE BIOCHEMICAL ENGINEERING. Continental Press Pte Ltd, 880 (751-753) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kazumori Funatsu: "Finite Element Method Analysis of 3‐D Flow in an Agitated Bioreactor" Cell Culture Engineering IV abstract. 117 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Keiichirou Fukushima: "Estimation of a shear stress history of the animal cella in an agitated bioreactor" 化学工学会第27回秋季大会研究発表講演要旨集. 第3分冊. 180 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuhiko Kawakubo: "T‐PA production by high density culture of weakly adherent human embryonic kidney cella using polyurethane foam packed‐bed culture" Applied Microbiology and Biotechnology. 41. 413-418 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 福島圭一郎: "三次元流動シミュレーションによる攪拌槽型バイオリアクターの最適設計に関する検討" 化学工学会第59年会研究発表講演要旨集. 第2分冊. 32 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Taku Matsushita: "Finite Element Analysis of 3‐Dimensional Flow in an Agitated Bioreacter" 化学工学シンポジウムシリーズ. (発表予定). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazomori Funatsu: "Finite Element Analysis of 3‐D Flow in an Agitated Bioreactor" Gytotechnology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松下 琢: "バイオプロセスシステム工学 -第6章 動物細胞培養-" 株式会社アイピーシー, 687 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazomori Funatsu: "BETTER LIVING THROUGH INNOVATIVE BIOCHEMICAL ENGINEERING" Continental Press Pte Ltd, 880 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山下和彦: "攪拌槽内三次元流動シミュレーションによるバイオリアクターの検討" 化学工学会第58年会研究発講演要旨集. 第3分冊. 9- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuhiko Kawakubo: "FEM analysis of 3-D flow in an agitated bioreactor" 化学工学会第26回秋季大会研究発表講演要旨集. 第3分冊. 87- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 川久保康彦: "三次元流動シミュレーションによる攪拌槽型バイオリアクターの設計" 第71期日本機械学会全国大会講演論文集. A. 237-239 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuhiko Kawakubo: "FEM Analysis of 3-D Flow in an Agitated Bioreactor" Japanese Association for Animal Cell Technology '93 Abstract. 54- (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuhiko Kawakubo: "T-PA production by high density culture of weakly adherent human embryonic kidney cells using polyurethane foam packed-bed culture" Applied Microbiology and Biotechnology. (発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松下 琢: "バイオプロセスシステム工学 -第6章 動物細胞培養-" 株式会社アイピーシー, 500 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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