Construction of Monomolecular Membrane with Different Surface Properties and Electron Transfer
| Project/Area Number |
05650930
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | Waseda University |
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Principal Investigator |
TAKEOKA Shinji Waseda University, Assistant Professor, 理工学部, 専任講師 (20222094)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Molecular Assembly / Surface Property / Amphiphilic Molecules / Vesicles / Molecular Orientation / Monomolecular Membrane / Hemoglobin / Electron Transfer |
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| Research Abstract |
Monomolecular membrane having different surface properties between inner and outer surface was prepared as vesicles. The purpose of this research is to study the characteristics of this molecular assemblies and to construct functional vesicles encapsulating concentrated protein such as hemoglobin. Amphiphilic molecules of which both ends are different head groups were dispersed into water by sonication to prepare vesicles of ca.100 nm with monomolecular layr. This was confirmed from TEM observation, the measurement of the total volume of the vesicles in comparison with calculated volume, and the shift of choline methyl proton peak in ^1H-NMR by the addition of Eu^<3+> to outer aqueous phase. The resulting vesicles were very stable, and no change of vesicular size and no leakage of aqueous contents were observed. The orientation of the amphiphiles in the membrane was regulated if the size was reduced or if concentrated hemoglobin was encapsulated. Electron transfer through the membrane was analyzed from the reduction of inner oxidants by adding reductant to the outer aqueous phase.
A lithocholic acid derivatives with ionic groups at both ends was incorporated to the phospholipid membrane. A significant influence of it on the molecular packing and the stability enhancement of the vesicle suspension were clarified by a fluorescence depolarization method, 1H-NMR,and a turbidity measurement.
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Report
(3 results)
Research Products
(10 results)
