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Structure and Function of Genes Involved in Insect Transmission of Rice Ragged Stunt Virus.

Research Project

Project/Area Number 05660040
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionHokkaido University

Principal Investigator

UYEDA Ichiro  Faculty of Agriculture, Hokkaido University, associate Prof, 農学部, 助教授 (10113523)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsRice ragged stunt virus / Insect vector / Genome segment
Research Abstract

1) When the genomic dsRNAs were electrophoresed, two types of genome segment 9 (S9) were detected by the mobility shift.The faster and slower migrating bands were designated as L and U,respectively.Although most of the isoates with L were not transmitted efficiently, some were in the same efficiency as those with U.
2) cDNAs to U and L were synthesized from the genomic dsRNAs, cloned into a plasmid vector pUC119, and sequenced.Both had the same length of 1132 nucleotides, therefore the mobility shift was not due to the differencein in length.L had some substitutions compared with U and the A to C convertion at a nucleotide position of 843 was common in all Ls.In order to prove this convertion results in the mobility shift, synthetic U and L having different nucleotides only at the position 843 were made.The electrophoretic analysis showed the convertion is responsible to the mobility shift.The substitution created the Asp to Ala convertion of the encoded polypeptide.
3) An isolate with L having an equal insect transmission rate was found to have another substitution at a nucleotide position 486 in addition to taht at the position 843.This change results in Asn to Ser convertion of the encoded polypeptide.
4) A polypeptide encoded by S9 was expressed in E.coli and antisera to the protein was made.In western blotting analyzes using the antibodies to the protein, only 38.6 K polypeptide located on a virion surface reacted positively among five structual polypeptides.
Above results indicated that 38.6K polypeptide located on the virion surface is involved in insect transmission interacting probably with receptors of an insect vector.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Uyeda et al.: "Nucleotide sequence of rice dwarf phytoreovirus genome segment 2:completion of sequence analyses of rice dwarf virus." Intervirology. 37. 6-11 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murao et al.: "Genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of variants of genome segment 12." Journal of General Virology. 75. 1843-1848 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yan et al.: "Echonochloa ragged stunt virus belongs to the same genus as rice ragged stunt virus." Ann. Phytopath. Soc. Japan. 60. 613-616 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uyeda et al.: "Rice ragged stunt onyzavirus genome segment 9 encodes a 38.6 KDa structural protein." Journal of General Virology. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suga et al.: "Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission" Arch. Virology. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yan et al.: "Molecular cloning and complete nucleotide sequence of rice ragged stunt oryzavirus genome segment 10." Ann. Phytopath. Soc. Japan. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uyeda et al.: "Nucleotide sequence of rice dwarf phytoreovirus genome segment 2 : Completion of sequence analyzes of rice dwarf virus." Intervirology. 37. 6-11 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murao et al.: "Genome heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequence of variants of genome segment 12" J.Gen.Virol.75. 1843-1848 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yan et al.: "Echinochloa ragged stunt virus belongs to the same genus as rice ragged stunt virus." Ann.Phytopath.Soc.Japan. 60. 613-616 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uyeda et al.: "Rice ragged stunt oryzavirus genome segment 9 encodes a 38.6 K Da structural ptotein." J.Gen.Virol.(In press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suga et al.: "Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segragation by insect vector transmission." Arch.Virol.(In press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yan et al.: "Molecular cloning and complete nucleotide sequence of rice ragged stunt oryzavirus genome segment 10" Ann.Phytopath.Soc.(In press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uyeda etal.: "Nudleotide sequence of rice dwarf phytoreovirus genome segment 2:completion of sequence analyses of rice dwarf virus." Intervirology. 37. 6-11 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Murao et al.: "Genomic heterogeniety of rice dwarf phytereovirus field isolates and nucleotide siquences of variants of genome segment 12." Journal of General Virology. 75. 1843-1848 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yan et al.: "Echonochloa ragged stunt virus belongs to the same genus as rice ragged stunt virus." Ann.Phytopath.Soc.Japan. 60. 613-616 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uyeda et al.: "Rice ragged stunt Oryzaviryus genome segment 9 encodes a 38.6 KDa structural protein." Journal of General Virology. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suga et al.: "Heterogeneity of rice ragged stunt Oryzaviryus genome segment 9 and its segregation by insect rector transmission." Arch.Virology. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yan et al.: "Molewlar cloning and complete nucleotide sequence of rice ragged Stunt Oryzaviryus genome segment 10" Ann.Phytopath.Soc.Japan. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.Azuhata: "Close Similarity Between Structures of Rice Black-Streaked Dwavf and Maize Rough Dwarf Viruses" Journal of General Virology. 74. 1227-1232 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] I.Uyeda: "Nucleotide Sequence of Rice Dwarf Phytoreovirus Genome Segment2:Completion of Sequence Analyses of Rice Dwarf Uirus" Intervirology. 37(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Murao: "Genomic Heterogeneity of Rice Dwarf Phytoreovirus Field Isolates and Nucleotide Sequence of Variants of Genome Sogment12" Journal of General Virology. 75(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 上田一郎: "イネ萎縮ウイルスの全遺伝子構造" 植物防疫. 47. 449-452 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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