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ANALYSIS OF THE REGULATION GENE FOR HYPERSENSITIIVITY OF PLANT AND ANALYSIS OF SIGNAL TRUNSDUCTION IN PLANT CELL

Research Project

Project/Area Number 05660047
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

FURUICHI Naotaka  NIIGATA UNIVERSITY AGRICULTURE ASSOCIATE PROFESSOR, 農学部, 助教授 (50238664)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsRESISTANCE / POTATO / PHYTOPHTHORA INFESTANS / ELICITOR / SUPPRESSOR / PROTON ATPASE / PROTEIN KINASE / ALTERNARIC ACID / ジャガイモ疫病菌 / 疫病菌 / プロトンポンプ / [H^+]ATPase / グルカン / 情報受容
Research Abstract

In vitro protein phosphorylation of the plasma membrane proteins of potato (Solanum tuberosum L.) tuber tissue and bean (Phaseolus vulgaris L.) hypocotyl tissue was stimulated by an hyphal wall (HWC) elicitor and suppressor (Mr. 4,700 and Mr 440) isolated from Phytophthora infestans. Phosphorylation levels were estimated at 25 muM free Ca^<2+>. For determination of ^<32>P-incorporation into [H^+] ATPase, phosphorylated proteins were separated by SDS-PAGE.^<32>P levels in the protein bands were analyzed by the ANBIS Radioanalytic Imaging System. The suppressor (250 mug/ml) stimulated phosphorylation of the plasma membrane [H^+]ATPase in potato more than the elicitor (250 mug/ml). Suppressor also stimulated the ATPase activity of bean plasma membranes.
Salicylic acid (15-30 muM) stimulated the phosphorylation level of potato and bean. In contrast, hydrogen peroxide decreased the protein phosphorylation level of both plants. Salicylic acid and hydrogen peroxide are potential chemical signals in plant defenses. We conclude that specific protein kinase(s) affect the P-type [H^+] ATPase. Regulation of protein kinase activity by fungal pathogen components may participate in membrane signal transduction for plant defense mechanisms.
Production of active oxygen group (superoxide radicals and hydrogen peroxide) in bean suspension cells were measured by the detectioin of chemiluminescence in cell suspension media. Hyphal wall elicitor caused genaration of active oxigen groups. In contrast, the suppressor of the fungus did not stimulate the production.
Hyphal wall elicitor (250 mug/ml) caused the production of active oxygen and hydrogen peroxide in potato suspension cells within 15 min, but the glucan of the fungus caused its production less than elicitor.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 古市尚高: "ジャガイモおよびインブンの過敏感反応における情報伝達機構" 植物微生物研究会(Proceeding). 3. 15-17 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古市尚高: "ジャガイモ疾病菌より抽出した核タンパク質エリシターの活性部位とその構造について" 植物微生物研究会(Proceeding). 3. 18-20 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.FURUICHI: "Specific phosphorylation of plasma membrane H^+-ATPase of potatc and bean cells stimulated by the fungel suppressor and elicitor of P.infestan" Annals of the phytophatholgical Society JAPAN. 59. 268 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.FURUICHI: "Phosphorylation of plasma membrane H^+-ATPase of potatc and bean cells stimulated by the fungel suppressor and elicitor of Phytophthora infestans" Proceedung of 6th International Congress of Plant Pitgology. 195 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.FURUICHI: "Membrane Signal Transduction of potato and bean defence reaction" Proceeding of the second Totlori International Symbosium on Host-Specific Toxin.17 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古市尚高: "植物細胞膜[H^+]ATPase活性に対する病原性決定因子アルタナル酸とその合成セグメントの作用" 植物微生物研究会報. 4. 23-25 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.FURUICHI: "Host-Specific Toxin:Biosynthesis,Rcceptor and Molecular Biology" Tottori University Press, 230 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "SIGNAL TRANSDUCTION OF POTATO AND BEAN CELLS TREATED WITH SUPPRESSOR AND ELICITOR OF PHYTOPHTHORA INFESTANS19GC01 : PROCEEDINGS OF THE III JAPAN PLANT MICROBE INTERACTION" VOL 3. 15-17 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "PURIFICATION AND PROPERTIES OF THE ELICITOR ISOLATED FROM PHYTOPHTHORA INFESTANS." PROFEEDINGS OF THE III JAPAN PLANT MICROBE INTERACTION. VOL 3. 18-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "SPECIFIC PHOSPHORYLATION OF PLASMA MEMBRANE H+-ATPASE OF POTATO AND BEAN CELLS STIMULATED BY THE FUNGAL SUPPRESSOR AND ELICITOR OF PHYTOPHTHORA INFESTANS." ANNALS OF THE PHYTOPATHOLOGICAL SOCIETY OF JAPAN. VOL 59. 268 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "PHOSPHORYLATION OF PLASMA MEMBRANE H+-ATPASE OF POTATO AND BEAN CELLS STIMULATED BY THE FUNGAL SUPPRESSOR AND ELICITOR OF PHYTOPHTHORA INFESTANS." PROCEEDINGS OF 6TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY. 195 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "MEMBRANE SIGNAL TRANSDUCTION OF POTATO AND BEAN DEFENCE REACTION." PROCEEDINGS OF THE SECOND TOTTOTI UNIVERSITY INTERNATIONAL SYMPOSIUM ON HOST-SPECIFIC TOXIN. 17 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: "EFFECT OF ALTERNARIC ACID AND ITS DERIVATIVES ON H+-ATPASE OF PLANT PLASMA MEMBRANE." PROCEEDINGS OF THE IV JAPAN PLANT MICROBE INTERACTION. VOL 4. 23-25 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] NAOTAKA FURUICHI: HOST-SPECIFIC TOXIN : BIOSYNTHESIS,RECEPTOR AND MOLECULAR BIOLOGY. TOTTORI UNIVERSITY PRESS, 1-230 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古市尚高: "ジャガイモにおよびインブンの過敏感反応における情報伝達機構" 植物微生物研究会. 3(Proceeding). 15-17 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古市尚高: "ジャガイモ疫病菌より抽出した糖タンパク質エリシターの活性部位とその構造について" 植物微生物研究会. 3(Proceeding). 18-20 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.FURUICHI: "Specific phosphorylation of plasma membrane H′-ATPase of potato and bean cells stimulated by the fungal suppressor and elicitor of P.infesta" Annals of the Phytopathological Society JAPAN. 59. 268- (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.FURUICHI: "phosphorylation of plasma membrane H′-ATPase of potato and bean cells stimulated by the fungal suppressor and elicitor of Phytophthre infestans" Procecding of 6th International Congress of Plant Pathology. 195- (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.FURUICHI: "Membrane Signal Transduction of potato and bean defence reaction" Proceeding of the second Tottori Umiversity International Synposium on Host-Specific Toxin.17- (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古市尚高: "植物細胞膜[H^+]ATPase活性に対する病原性決定因子アルタナル酸とその合成セグメントの作用" 植物微生物研究会報. 4. 23-25 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.FURUICHI: "Host-Specific Toxin:Biosynthesis,Rcceptor and Molecular Biology" Tottori University Press, 230 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 古市尚高: "ジャガイモおよびインゲンの過敏感反応における情報伝達機構" 植物微生物研究会. 3(Proceeding). 15-17 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 古市尚高: "ジャガイモ疫病菌より抽出した糖タンパク質エリシターの活性部位とその構造について" 植物微生物研究会. 3(Proceeding). 18-20 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.FURUICHI: "Specific phosphorylation of plasma membrane H^+-ATPase of potato and bean cells stimulated by the fungal suppressor and elicitor of P.infestans" Annals of the Phytopathological Society JAPAN. 59. 268 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.FURUICHI: "Phosphorylation of plasma membrane H^+-ATPase of potato and bean cells stimulated by the fungal suppressor and elicitor of Phytophthora infestans" Proceeding of 6th International Congress of Plant Pathology. 195 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.FURUICHI: "Membrane Signal Transduction of potato and bean cells in defence reaction" Proceeding of the second Tottori University International Symposium on Host-Specific Toxin.17 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.FURUICHI: "Host-Specific Toxin:Biosynthesis,Receptor and Molecular Biology" Tottori University Press, 230 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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