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Studies on the signal transduction network in adaptive responses in microorganisms.

Research Project

Project/Area Number 05660097
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KATO Junichi  Hiroshima Univ.Faculty of Engineering, Assistant Prof., 工学部, 助手 (90231258)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsAdaptive responses / Signal transduction / Information processing / Microorganism / Chemotaxis / Pho regulon / Phoレギュロン / シグナル伝達系
Research Abstract

The final goal of our research is to understand the signal transduction networks in a bacterial cell.We have focused on the signal transductions in the adaptation response system for phosphate starvation in Pseudomonas aeruginosa for two years and performedthe genetic analyzes of the Pi taxis and the phosphate regulon.The summary of research results is as follows :
1.An constitutive alkaline phosphatase (AP) mutant, designated APC1, showed a constitutive phosphate (Pi) taxis.The result suggests the existence of a common negative regulator for the Pi regulon and the Pi taxis.
1.A chromosomal phoB mutant still showed the inducible Pi taxis, indicating the Pi taxis is not regulated by the phoB gene unlike the Pi regulon.
2.We cloned a 27-kb DNA fragment that complements the mutation of APC1.Genetic analyzes of the fragment revealed the negative regulator is the Pi specific transport channel complex.
4.A 20-kb fragment was obtained by shot-gun cloning usibg a chemotaxis deficient mutant as a host strain.Two chemotaxis genes, cheY and cheZ were found on the fragment by genetic analyzes.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] HARDOYO: "Bacterial motility as a potential tool for rapid toxicity assays" Biotechnique. 7. 457-462 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomomi OHGA: "Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa" FEMS Microbiol.Lett.113. 63-66 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in tne negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Agus MASDUKI: "Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa" J.Bacteriol.177. 948-952 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 加藤純一: "バクテリアのリン酸獲得系をめぐる情報伝達経路" Bulletin of Japanese Society of Microbial Ecology. 8. 183-189 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大竹久夫: "細菌細胞のソフトウエア" バイオサイエンスとバイオインダストリー. 52. 11-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO: "Phosphate in Microorganisms:Cellular and Molecular Biology" American Society for Microbiology, 347 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO: "Better Living Through Innovative Biochemical Engineering" Continental Press, 880 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hisao OHTAKE: "Trends in Microbial Ecology" Spanish Society for Microbiology, 717 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] HARDOYO,Shunsuke KAJIWARA,Junichi KATO,and Hisao OHTAKE: "Bacterial motility as a potential tool for rapid toxicity assays" Biotechnique. 7(7). 457-462 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomomi OHGA,Agus MASDUKI,Junichi KATO,and Hisao OHTAKE: "Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa" FEMS Microbiol.Lett.113(1). 63-66 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO,Yukihiro SAKAI,Toshiyuki NIKATA,and Hisao OHTAKE: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis" J.Bacteriol. 176(18). 5874-5877 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Agus MASDUKI,Jin NAKAMURA,Tomomi OHGA,Ryuichi UMEZAKI,Junichi KATO,and Hisao OHTAKE: "Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa" J.Bacteriol.177(4). 948-952 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO,and Hisao OHTAKE: "Signal transduction pathways in the assimilation system for phosphate in bacteria" Bulletin of Japanese Society of Microbial Ecology. 8(3). 183-189 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hisao OHTAKE,Junichi KATO,and Akio KURODA: "Software in a bacterial cell" Bioscience and Biotechnol.52(9). 11-16 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO,Yukuhiro SAKAI,Toshiyuki NIKATA,Agus Masduki, and Hisao OHTAKE: Phosphate taxis and its regulation in Pseudomonas aeruginosa. In : A.Torriani-Gorini, E.Yagil and S.Silver(eds.)"Phosphate in Microorganisms : Cellular and Molecular Biology", American Society for Microbiology, 315-317 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junichi KATO,Yukuhiro SAKAI,Kazunori TAGUCHI,Agus MASDUKI,Hisao OHTAKE: Genetic control of chemosensing for inorganic phosphate in Pseudomonas aerugiosa. In : W.K.Teo, M.G.S.Yap and S.K.W.Oh(eds)"Better Living Through Innovative Biochemical Engineering", Continental Press, 505-507 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hisao OHTAKE,Junichi KATO,and Toshiyuki NIKATA: Bacterial sensory transduction to detect changing environment. In : R.Guerrero and C.Pedros-Alio (eds.) "Trends in Microbial Ecology"Spanish Society for Microbuology, 101-104 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Agus Masduki: "Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa." J.Bacteriol.177. 948-952 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junichi Kato: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junichi Kato: "Phosphate in microorganisms:Cellular and Molecular" American Society for Microbiology, 347 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junichi Kato: "Better Living Through Innovative Biochemical Engineering" Continental Press, 880 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tomomi Ohga: "Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa" FEMS Microbiol.Lett.113. 63-66 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Junichi Kato: "Genetic control of chemosensing for inorganic phosphate in Pseudomonas aeruginosa." Proceedings of the Pho93 symposium. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤純一: "バクテリアのリン酸獲得系をめぐる情報伝達経路" Bulletin of Japanese Society of Mirobial Ecology. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hisao Ohtake: "Bacterial Sensory transduction to detect changing environments in Trends in Microbial Ecology" Spanish Society for Microbiology, (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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