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Search for fat-soluble ligand of nuclear orphan receptor.

Research Project

Project/Area Number 05660106
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionTokyo University of Agriculture

Principal Investigator

KATO Shigeaki  Tokyo Univ.of Agriculture, Dep.of Agricultural Chemistry, Associate Professor, 農学部, 助教授 (60204468)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsnuclear receptor / fat-soluble ligand / gene expression / transcriptional control / vitamin D / vitamin A / orphan receptor / 脂溶性生理活性物質
Research Abstract

Steroid/thyroid/vitamin A,D nuclear receptors form a gene superfamily, and they resemble each other in terms of structure and function. Besides of the nuclear receptors, whose ligands have been found, receptors with unknown ligands exist as "orphan receptor" . When the ligand for orphan receptor will be identified, a new signaling pathway becomes clear. Moreover, a cross-talk with the signaling pathways for fat-soluble ligands will be clarified.
In the present study, the subtypes and isoforms for vitamin A receptors (RARs, RXRs) and vitamin D receptors (VDR) as well as orphan receptors have been searched with cDNAs encoding RAR,RXR and VDR from the cDNA libraries of various mammalian tissues. Consequently, we found a VDR isoform (VDR1) acting as a dominant negative receptor against the wild type of VDR (VDR0). Considering the presence of multiple active forms of vitamin D on biological actions, one of these compounds may act as a ligand for VDR1. In addition, the functional analysis of identified orphan receptors is under investigation.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] H.Sasaki,et al.: "Transcriptional activity of a fluorinated vitamin D analog on VDR-RXR mediated gene expression." Biochemistry. 34. 370-377 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] R.Kojima,et al.: "In vivo isomerization of retinoic acid:rapid isomer exchange and gene expression." J.Biol.Chem.269. 32700-32707 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Nagasaki,et al.: "Vitamin A regulates the expression of apolipoprotein AI and CIII genes in the rats." Biochem.Biopys.Res.Commun.205. 1510-1517 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Ohyama,et al.: "Identification of a vitamin D-responsive element in the 5'-flanking region of the rat 25-hydroxy vitamin D3 24-hydroxylase gene." J.Biol.Chem.269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Mano,et al.: "Positive and negative regulation of retinoid X receptor gene expression by thyroid hormone in the rat." J.Biol.Chem.269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Okano,et al.: "Retinoic acid up-regulates erythropoietin production in hepatoma cells and vitamin A-depleted rats." FEBS lett.349. 229-233 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 加藤茂明: "核内レセプターと情報伝達" 実験医学バイオサイエンス 羊土社, 1-125(125) (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 加藤茂明: "受容体の分子生物学" 日本臨床 日本臨床社, 17-23(7) (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Sasaki, et al.: "Transcriptional activity of a fluorinated vitamin D analog on VDR-RXR mediated gene expression." Biochemistry. 34. 370-377 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] R.Kojima, et al.: "In vivo isomerization of retinoic acid : rapid isomer exchange and gene expression." J.Biol.Chem.269. 32700-32707 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Nagasaki, et al.: "Vitamin A regulates the expression of apolipoprotein AI and CIII genes in the rats." Biochem.Biopys.Res.Commun.205. 1510-1517 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Ohyama, et al.: "Identification of a vitamin D-responsive element in the 5'-flanking region of the rat 25-hydroxy vitamin D3 24-hydroxylase gene." J.Biol.Chem. 269. 10545-10550 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Mano, et al.: "Positive and negative regulation of retinoid X receptor gene expression by thyroid hormone in the rat." J.Biol.Chem.269. 1591-1594 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Okano, et al.: "Retinoic acid up-regulates erythropoietin production in hepatoma cells and vitamin A-depleted rats." FEBS lett.349. 229-233 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Mano: "Positive and negative regulation of retinoid X receptor gene expression by thyroid hormone in the rat" J.Biol.Chem. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Ohyama: "Identification of a vitamin D‐responsive element in the 5'‐flanking region of hte rat 25‐hydroxyvitamin D3 24‐hydroxylase gene" J.Biol.Chem. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kato: "Gene expression of cellular retinol‐binding protein l(CRBPI)is affected by dietary proteins in rat liver" J.Nutr.Sci.Vitaminol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kato: "ldentification of nuclear factor interacting with a retinoic acid respomse element in the rat nucleus" J.Nutr.Sci.Vitaminol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Mano: "Thyroid hormone affects the gene expression of retinoid X receptors in the adult rat" Biochem.Biophys.Res.Commun.191. 943-949 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kamei.: "Vitamin D receptor gene expression is up‐regulated by 1,25‐dihydroxyvitamin D3 in 3T3‐L1 preadipocytes" Biochem.Biophys.Res.Commun.193. 948-955 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤茂明: "受容体タンパク質〓、13章ステロイドホルモン型受容体" 廣川書店, 391-446 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加藤茂明: "新生化学実験講座9 ホルモン〓、15章レチノイン酸" 東京化学同人, 281-304 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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