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Chemistry and functions of novel type of petide antimutagenic factors produced by microorganisms

Research Project

Project/Area Number 05660116
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionNagoya University

Principal Investigator

OSAWA Toshihiko  Nagoya University Department of Applied Biological Sciences Associate Professor, 農学部, 助教授 (00115536)

Co-Investigator(Kenkyū-buntansha) UCHIDA Koji  Nagoya University Department of Applied Biological Sciences Assistant Professor, 農学部, 助手 (40203533)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsAntimutagenic factor / Streptomyces / Peptide / Antimutagens / E.coli / E.coli MP-1 / E.coli WP2s / 微生物代謝産物 / 大腸菌MP-1
Research Abstract

In order to obtain novel type of antimutagenic factors produced by Streptomyces sp.using E.coli MP-1 and WP2s strains as the bioassay systems. Purification and isolation of active compound by column chromatogrphy and preparative HPLC from the metabolites of Streptomyces SP AJ 9455, two main antimutagenic compounds were isolated. One of antimutagenic components, BA-2, has been identified as N-methyl-valyl-amycleomycin. Antimutagenic mechanism has been investigated using several E.coli strains and it was found that BA-2 has strong inhibitory effects on SOS mutagenesis. Other antimutagenic factor which was named BA-1 supposed to be polypeptide whose molecular weight is 7000-10000, however, BA-1 has been speculated as small molecule peptide type antimutagen. Structure analyzes have been carried out using NMR,MS,IR and also aminoacid analysis, and it was found BA-1 has molecular weight of 436 although other detailed structre is still unknown. Now, structure analyzes and investigation of antimutagenesis mechanism of BA-1 are underway.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M.Shiraki: "Antioxidative and Antimutagenic effects of the aflavims from black teas." Mutation Res.323. 29-34 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Nakayama: "Suppression of hydrogen perxide-induced cytotoxicity toword chinese hamgter lung tibroblasts by decylubiquinone,a coenzyme Q analog" Biosci.Bioteck.Biochem.58. 1702-1704 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yamada: "Antimutagenic effects of N-methyl-valyl-amicleomycin (BA-2)isolated from the metabolites of Streptomyees sp." Mutation Res.,. 286. 293-297 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Uchida: "2-Oxo-histidine as a novel biological marker for oxidatively madified proteins" FEBS Letters. 332. 208-210 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 大澤俊彦: "がんを防ぐ52の野菜" 法研(東京), 180 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Shiraki et al.: "Antioxidative and antimutagenic effects of theaflavins from black teas" Mutation res.323. 29-34 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Nakayama et al.: "Suppression of hydrogen peroxide-induced cytotoxicity toward Chinese hamster lung fibroblasts by decylubiquinone" a Coenzyme Q analogue, Biosci.Biotech.Biochem.58. 1702-1704 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Uchida et al.: "Identification of oxidized histidine generated at the active site of Cu, Zn-superoxide dismutase exposed to H_2O_2, Selective generation of 2-oxo-histidine at the histidine-118" J.Biol.Chem.269. 2405-2410 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Osawa, T.: "Plant antioxidants ; Protective role against oxygen radical species" Cosmetics and Toiletries magazine. 109. 77-81 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Uchida et al.: "Quantification of 4-hydroxy-nonenal protein adducts" Method Enzymol.233. 371-380 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Tsuda.: "Antioxidative activity of the anthocyanin pigments cyanidin-3-0-β-D Glucoside and cyanidin" J.Agric.Food Chem.42. 2407-2410 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Uchida: "Identification of oxidiged histidine generated at the active site of Cu,Zn-superaxide dismutase exposed bo H_2O_2.Selective generation of 2-Oxo-his at the his-118." J.Biol.Chem.269. 2405-2410 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yamada: "Antimutagenic effects of N-methyl-Valyl-amiclenomycin(BA-2)isolated from the metabolites of Streptomyces sp." Mutation Research. 286. 293-297 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Uchida: "2-Oxo-histidine as a novel biological marker for oxidatively modified proteins" FEBS Lett.332. 208-210 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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