| Project/Area Number |
05660136
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
食品科学・栄養科学
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
HORIO Fumihiko Nagoya University, School of Agricultural Sciences, Department of Applied Biological Sciences, Associate Professor, 農学部, 助教授 (20165591)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Ascorbic acid / Apolipoprotein A-I / Atherosclerosis / Vitamin C |
|---|
| Research Abstract |
Apolipoprotein A-I (Apo A-I) is a major apolipoprotein of serum high density lipoprotein (HDL) , which transports cholesterol from peripheral tissues to liver. We observed that apo A-I in serum HDL was decreased in ascorbic acid (AsA) deficiency in scurvy-prone ODS-od/od rats. In this study, we examined the mechanism how AsA deficiency causes a low level Apo A-I in serum.
The Apo A-I mRNA level in the liver but not in the small intestine was decreased (50%) in AsA-deficient group compared to in the control group. As AsA deficiency did not affect the transcription rate of Apo A-I gene, AsA might be required to stabilize Apo A-I mRNA.Moreover, a lower level of albumin mRNA,a higher level of haptoglobin mRNA and a higher level of 1-acid glycoprotein mRNA in the liver was observed in ascorbic acid-deficienct rats compared to in the control rats. These changes in hepatic levels of mRNA including Apo A-I mRNA are also observed in acute phase or inflammation. At present, we are measuring the serum levels of interleukin-6, which is a major cytokine secreted in acute phase and inflammation, in ascorbic acid deficiency.
|
