| Project/Area Number |
05660165
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
林学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
TOGASHI Katsumi Hiroshima Univ, Fac.Integrated Arts and Sci., Asso.Prof., 総合科学部, 助教授 (30237060)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Keiichi Hiroshima Univ.Fac.Integrated Arts and Sci., Asso.Prof., 総合科学部, 助教授 (00238809)
HOROKOSHI Takao Hiroshima Univ.Fac.Integrated Arts and Sci., Prof., 総合科学部, 教授 (00094102)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Pine wilt disease / Susceptibility / Pinus densiflora / Pinus thunbergii / Allelopathy / Co-occuring tree species / Monochamus alternatus / Bursaphelenchus xylophilus |
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| Research Abstract |
To determine the effect of allelochemicals of Alnus sieboldiana on the susceptibility of Pinus trees to pine wilt disease.P.thunbergii seedings were cultured with the solution which was extracted from air-dried leaves and twigs of A.sieboldiana and Eurya japonica with water.The solution used was diluted at 1,10^<-1>,10^<-2> or 10^<-3>.
Bursaphelenchus xylophilus was inoculated on seedlings a week later.Mortality from pine wilt was higher on A.sieboldiana extract than on E.japonica at 10^<-3>.
To identify the allelochemicals of A.sieboldiana, methanol extract of the leaves was fractionated.Aqueous fraction extracted with i-BuOH inhibited the root growth of lettuce and made a terminal part of root brown at concentrations of 500 and 1,000 ppm.^3C-NMR spectrum and bioassay showed that D-(+)-catechin was an allelochemical.The inhibitory effect on root growth was induced by catechin itself and not by metabolites of catechin by root.
To clarify the effect of A.sieboldiana on the reproduction of insert vector of pine wilt, Monochamus alternatus adults were raised in outdoor cages containing A.sieboldiana or Eurya japonica potted.Cages excluding both tree species were served as control.There were no difference among adult females in survivorship and fecundity.
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