| Project/Area Number |
05660223
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHINANO Haruo Faculty of Fisheries, Hokkaido University, Professor, 水産学部, 教授 (10001603)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Koji Faculty of Fisheries, Hokkaido University, Assistant, 水産学部, 助手 (40250500)
KAWAI Yuji Faculty of Fisheries, Hokkaido University, Lecturer, 水産学部, 講師 (60195039)
INOUE Norio Faculty of Fisheries, Hokkaido University, Professor, 水産学部, 教授 (60002086)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Squid / Shiokara / Microflora / Antibacterial substances / Staphyococcus aureus / Squid liver / Squid ink |
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| Research Abstract |
The effect of various concentrations of the addition of squid liver and to "Ika-Shiokara" with 10% salt were examined on the changes of microflora and chemical prooeties of "Ika-Shiokara" and the inoculated pathogenic organism, Staphylococcus aureus.
In the case of the "Ika-Shokara" without the addition of squid liver, the dominant species of S.warneri found in the early stage (2-4days) were replaced by Micrococci in the later stage of ripening.In the case of the sample with 1% squid liver, the dominant microorganism was S.warneri in the early stage, and the Staphylococci including S.warneri were replaced by Micrococci during the later stage of ripening (4-14days).In the case, the quility of "Ika-Shiokara" was decreased with the shift of dominant Staphylococci to Micrococci.In the case of 3% liver addition, among the dominant Staphylococci over the ripening preocess, S.epidermidis were shown to predominated on the last day of sampling.The inoculated S.aurues decreased during the ripening process with the addition of aquid liver 3-6%.The squid ink had a particulaly strong effect, and elongated the period of edibility.
S.epidermidis has never been isolated from "Ika-Shiokara" with the addition of ink, and was therefore isolated without ink.In the in vitro experiments, both S.epidermidis and S.aureus were inhibited by the extracts of heated squid ink.These findings suggest that the addition of squid liver and ink plays important roles in the formation of specific microflora of " Ika-Shiokara", and there are heat stable inhibitory substances in the squid liver and ink.
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