| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
1. In vivo lipid peroxidation levels and antioxidant activities in the liver and plasma of cultured yellowtail Seriola quinqueradiata were compared with those of wild fish. Results obtaianed suggest that cultured fish may suffer from more oxidative stress than wild fish and that alpha-tocopherol and ascorbic acid supplements are necessary in the diets of yellowtail to prevent the progress of in vivo lipid peroxidation which brings about many diseases.
2. Hyperbilirubinemia occurred in coho salmon, Oncorhynchus kisutch, infected with erythrocytic inclusion body syndrome (EIBS) virus. Secretory disorder of bilirubin occurred in the diseased fish was one of the causes of hyperbilirubinemia observed in coho salmon infected with EIBS virus.
3. Biliverdin being a diazo negative substance in vitro become a diazo positive sustance in plasma or bile. Therefore bilirubin level in fish plasma in which biliverdin exists must not analyzed by diazo method.
4. Yellow tail Seriola quinqueradiata were fed
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on extruder diets (EP) with different alpha-tocopherol (alpha-Toc) level. Reverse relation was observed between alpha-Toc contents and 2- thiobarbituric acid reactive substances (TBARS) values. Although ascorbic acid level of each diet was the same, the levels in the liver fed on diet containing higer level of alpha-Toc was higer than those containing lower level of alpha-Toc.
5. Linear correlation was observed between TBARS values and bilirubin content in the plasma injected with bacteria which is thought to be one of the cause of jaundice. However, no linear correlation was observed between bilirubin contents and bacteria number.
6. Seven bilirubin metabolites negative to the diazo reaction were identified in the urine of healthy persons by enzyme-linked immunosorbent assay (ELISA) using the anti-bilirubin monoclonal antibody 24G7. Two of the seven metabolites were isolated and their chemical structures were determined using fast-atom bombardment-mass spectroscopy (FAB-MS) and 1H-NMR.The two metabolites are 1,14,15,17-tetrahydro-2,7,13-trimethy1-1,14-dioxo3-vinyl-16H-tripyrrin-8,12-dipropionic acid and 1,14,15,17-tetrahydro-3,7,13-trimethy11,14-dioxo-2-vinyl-16H-tripyrrin-8,12-dipropionic acid. Less
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