| Project/Area Number |
05660309
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Mie University |
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Principal Investigator |
GOTO Masakazu Fac.Bioresouces, Mie Univ.Associate Professor., 生物資源学部, 助教授 (20144230)
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| Co-Investigator(Kenkyū-buntansha) |
EHARA Hiroshi Fac.Bioresouces, Mie Univ.Assistant Researcher., 生物資源学部, 助手 (10232969)
MORITA Osamu Fac.Bioresouces, Mie Univ.Associate Professor., 生物資源学部, 助教授 (90024562)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | ammonia treatment / rice straw / cell wall structure / UV spectrometry / cellulose crystallinity / SEM and TEM observations / lignin structure / luminal degradability / 細胞壁構造 / X線回析 / セルロース結晶化度 / リグニン / 繊維質消化率 |
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| Research Abstract |
The objective of this study was to investigate changes in chemical and structural features of the cell walls in barley (Hordium vulgare L.) and rice (Oryza sativa L.) following treatment with ammonia (30g NH_3 kg^<-1> straw) and to relate to the improvement in cell wall degradability by rumen micrrorganisms.
Stem specimens of untreated and ammonia-treated straw were prepared for SEM and TEM observations. Water- and methanol-extracts of finely ground stem samples were subjected to IR spectrometry and appropriate chemical analysis. The X-ray diffraction patterns, water-holding capacity and swollen volume were also examined.
Changes in chemical composition of barley straw following the treatment were seen as an increase in the nitrogen content of the cell wall fraction and as a partial loss of ester-linked phenolic acids and acetyl groups. Ammonia treatment promoted access by rumen microorganisms to the luminal surface of parenchyma and sclerenchyma cells apparently due to an alteration in the fragility of a thinner, rigid layr covering the inner surface of cell walls. Two measures of the X-ray diffraction pattern in treated straw indicated that treatment of barley straws produced a significant reduction in crystallinity index values of 14-24%. The water retention of the treated barley straw was 30% greater than that of the untreated straw following centrifugation at 300g and 3000g. A slightly higher swollen volume was also observed for water-saturated ammonia-treated barley straw, although the difference was not significant. It is suggested that two major consequences of treatment, the effect of ammonia as weak base on ester bonding within the cell wall and the ability of ammonia in an undissociated form to affect cellulose crystallinity, combine to increase the degradability of the treated straw.
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