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Practical study of microsatellite polymorphisms

Research Project

Project/Area Number 05660319
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionHokkaido University

Principal Investigator

UEDA Junji  Faculty of Agriculture, Hokkaido University, Associate Prof., 農学部, 助教授 (50002374)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsLivestock / Pig / DNA / Microsatellite / PCR / Polymorphism / DNA多型 / 遺伝的変異
Research Abstract

Microsatellite being highly polymorphic have been suggested for useful markers to make linkage may in farm animals.Positive clones with microsatellite in swine genome were identified by hybridization with a non-isotopic(DIG) labelled probes inclucing murine (Ca)_n and swine (GT)_n repeat sequences which were amplified by PCR.Four microsatellites were detected by this method.Two pairs of primers were synthesized (MPH7F : 5'GATCTTTCCACTTAACCTTTTTGTC, MPH7R : 5'TTTATCATTGTAAGACAGATTTGTA ; MPH9F : 5'GGCAAACATTCAGACCAGAG,MPH9R : 5'GAATCTGTCCATAATCAAGG).Electrophoretic variations of these microsatellites were determined by these of polyacrylamide nondenaturing gels and ethidium bromide staining under exposure to UV radiation.This work clarified the non RI method of microsateilite cloning and detection procedures.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] 紺屋直樹.長南史男: "肥料価格安定の経済効果" 農経論叢. 51. 189-198 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary

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Published: 1993-04-01   Modified: 2016-04-21  

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