• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Secretion and gene expression of ovarian hormone in the chicken

Research Project

Project/Area Number 05660326
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionNippon Veterinary and Animal Science Niversity (1994)
Nagoya University (1993)

Principal Investigator

TOMOGANE Hiroshi  Nippon Veterinary and Animal Science University, Department of Animal Science, Associate Professor, 獣医畜産学部, 助教授 (30023493)

Co-Investigator(Kenkyū-buntansha) SHIMADA Kiyoshi  Nagoya University, Department of Animal Science, Professor, 農学部, 教授 (40065579)
蛭薙 観順  名古屋大学, 農学部, 助手 (00126898)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsP450_<17>alpha_<hydroxylase> / P450aromatase / mRNA / sexual differentiation / ovary / testis / estradiol / arginine vasotocin / ニワトリ / シクロオキシゲナーゼ / ノーザンハイブリダイゼーション / プロスタグランジン / 卵巣 / 卵胞膜内細胞 / 卵胞膜外細胞 / 顆粒膜細胞 / テストステロン / エストラジオール / P450アロマターゼ / 遺伝子発現
Research Abstract

Study(1). Changes in messenger ribonucleic acid (mRNA) levels of P450_<17>alpha and P450arom of the ovary and testis in the chicken embryos. Since P450aromatase (P450arom) is a key enzyme converting androgen to estrogen, gene expression of P450arom during an early development of chick embryos seems important. In this study mRNA levels of P450_<17>alpha and P450arom of the ovary and testis were determined during the period between day 10 of incubation and day of hatching and the change were correlated with those in gonadal estradiol levels. P450_<17>alpha mRNA levels were higher in the left ovary than in the testis between day 10-14 of incubation but thereafter there was no difference between the left ovary and testis. P450arom mRNA levels wre always higher in the left ovary than in the testis and these changes were paralleled to these of estradiol. The results indicate that higher production of the ovarian estradiol in earlier stage in reflected by mRNA levelds of P450arom of the ovary … More . Study (2). Localization of P450arom mRNA in the gonad in relation to sexual defferentiation in the chicken. In situ hybridization was performed in the gonad of developing chick embryos using P450arom riboprobe. P450arom mRNA was not detected until day 6 of incubation but was observed after day 6.5 in the genetic female gonad, whereas no mRNA was observed in the genetic male gonad. Study (3). Gene expression of arginine vasotocin in the ovary of the laying hen. Using reverse trascription-polymerase chain reaction (RT-PCR) method, mRNA levels of arginine vasotocin were determined in the conpartmentalized cells of the ovarian follicles. During follicular maturation the mRNA levels of the granulosa decreased, but there was no change in the theca interna. Theca externa showed a significant reduction at the second largest follicle but increased at the largest. These results indicate that gene expression of arginine vasotocin in ovarian cells may by regulated differntially during folliclular growth in the chicken. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tomogane,H.: "A factor(s)from a rat trophoblast cell line lnhibits prolaction secretion in vivo and in vitro" Biology of Reproduction. 48. 325-332 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohkubo,T.: "High-level expression of biologically active chucken prolactin in E.coli." Comparafive Biochemistry and Physiology. 105. 123-128 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Pituitary and Plasma arginine vasotocin levels in the lamprey,Lamperta japonica." Comparative Biochemistry and Physiology. 107. 23-26 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 神作宜男: "チャボの就巣に伴う下垂体ホルモン遺伝子発現" 東海畜産学会誌. 4. 38-41 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sechman,A.: "Tissue-specific expression of calbindin-D_<28R>gene during ontogency of the clucken" Journal of Experimental Zoology. 269. 450-457 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 島田清司: "産卵制御の分子機構" 学術月報. 印刷中. 印刷中 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada,K.: "The molecular biology of prolactin in “Avian Endocrinology"" Sharp,J.K., 13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomogane, H.: "A factor(s) from a rat trophoblast cell line inhibits prolactin secretion in vivo and in vitro." Biol.Reprod.48-2. 325-332 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohokubo, T.: "High-level expression of biologically active chicken prolactin in E.coll." Comp.Biochem.Physiol.105-1. 23-26 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uchiyama, M.: "Pituitary and plasma arginine vasotocin level in the lamprey, Lamperta japonica." Comp.Biochem.Physiol.107-1. 23-26 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kansaku/Nobuo: "Chabo no shuso ni tomonau kasuitai horumon idenshi hatsugen" Toukai tikusan gakkaiho. 4-1. 38-41 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sechman, A.: "Tissue-specific expression of calbindin-D_<28k> gene during outogency of the chicken" J.Expt.Zool.269-5. 450-457 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada/Kiyoshi: "Sanran seigyo no bunshi kiko" Gakujutsu geppo. Insatutyu. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada, K.: "The molecular bilogy of prolactin in Avian Endocrinology" Sharp, P.J., ed.J.Endocrinol.Ltd., Bristol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomogane,H.: "A factor(s) from a rat trophublast cell line inhibits prolaction secretion in vivo and in vitro." Biology of Reproduction. 48. 325-332 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohkubo,T.: "High-level expression of biologically active chicken prolactin in E coli." Comparative Biochemistry and Physiology. 105. 123-128 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uchiyama,M.: "Pituitary and plasma arginine vasotocin levels in the lamprey Lamporta japonica." Comparative Biochemistry and Physiology. 107. 23-26 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 神作 宜男: "チャボの就巣に伴う下垂体ホルモン遺伝子発現" 東海畜産学会報. 4. 38-41 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sechman,A.: "Tissue-specific expression of calbindin-D28R gene during ontogeny ofthe chicken" Journal of Experimental Zoology. 269. 450-457 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島田 清司: "産卵制御の分子機構" 学術月報. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimada,K.: "The molecular biology of prolactin in "Avian Endo-crinology"" Sharp,P.J., 13 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohkubo,T.,Tanaka,M.,Nakashima,K.,Shimada,K.: "High-level expression of biologically activechicken prolactin in E.coli." Comparative Biochemistry & Physiology. 105A. 123-128 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Uchiyama,M.,Saito,N.and Shimada,K.: "Pituitary and plasma arginic vasotocin levels in the lamprey,Lamperta japonica." Comparative Biochemistry & Physiology. 107A. 23-26 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tomogane,H.et al.: "A factor(s) from a rat trophoblast cell line inhibits prolactin secretion in vitro and in vivo." Biol.Reprod.48. 325-332 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.,Shimada,Ohkubo,T.,Saito,N.,Talbot,R.: "Avian Endocrinology" Journal of Endocrinology Ltd., 13 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] N.,Saito,Shimada,K.and Koike,T.I.: "Avian Endocrinology" Journal of Endocrinology Ltd., 8 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi