| Project/Area Number |
05660327
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YAMADA Masayasu Kyoto Univ., Faculty of Agri., Associate Professor, 農学部, 助教授 (10243073)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Yoshihiko Kinki Univ., Res.Inst.of Bio-Oriented Sci.and Tech., Instructor, 生物理工学部, 講師 (70192739)
MINAMI Naojiro Kyoto Univ., Faculty of Agri., Researcher, 農学部, 助手 (30212236)
UTSUMI Kyozo Kyoto Univ., Faculty of Agri., Professor, 農学部, 教授 (90033266)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | preimplantation / embryo / in vitro development / PHA-P lectin / compaction / N-linled carbohydrates / oviduct extracts / ラット / 発生 / PHAレクチン / 卵管因子 / マウス / 糖鎖 / Nグリカナーゼ |
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| Research Abstract |
The developmental block at the 2-cell stage in rat embryos cultured in vitro has recently been overcome by deleting phosphate and glucose from culture medium. However, the problem was still remained that early 1-cell embryos were less able to develop in vitro to the blastocyst stage than 2-cell embryos. To improve the culture conditions for the 1-cell embryos, we first examined the effect of PHA-P lectin, which is known to induce in vitro maturation of mouse oocytes. As the results, it was found that PHA-P,especially E_4 subunit promoted the developmental ability of rat 1-cell embryos to blastocysts in vitro, suggesting that the action of PHA-P is specific and a modification of cell surface carbohydrate moieties of the embryos. Furthermore, we found that development of rat 1-cell embryos to blastocysts was promoted with macromolecules in rat oviduct extracts, whose molecular mass was about 70kDa.
Concerning the study on the involvement of Cell surface carbohydrates in compaction in the mouse embryos at the morula stage, it was revealed that cell surface N-linked carbohydrates that were specifically cleaved by N-glycanase participated greatly in the compaction process.
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