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Cytochemical study on the transitional regions between endoplasmic reticulum and Golgi apparatus

Research Project

Project/Area Number 05670009
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NODA Toru  Kyoto University, Department of Anatomy, Instructor, 医学部, 助手 (50156204)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGolgi apparatus / Endoplasmic reticulum / cytochemistry / small intestine
Research Abstract

Morphological and cytochemical characteristics of the transitional regions between endoplasmic reticulum (ER) and Golgi apparatus (GA) was investigated in duodenal epithelial cells. Recently machinary of protein transport between ER and GA has been well studied and most of all the hypotheses are based on vesicle transport. However, we found several morphological evidences which suggest other ways of substancial transport than vesicular carrier. The first evidence is cisternal transition from rough ER to Golgi cisternae, frequently observed in immature absorptive cells. In this type of transition, the cisternae of rough ER positioned on the trans face of GA approach and stack onto the trans-most cisterna losing their ribosomes on the way of stacking. Cytochemical labeling of the comparative cisternae by inosine diphosphatase (IDPase) which differentially labels ER and trans Golgi cisternae with light and heavy reaction products, respectivery, show different staining pattern in the same … More cisterna as being ER-type labeling away of Golgi stack and Golgi-type within the Golgi stack. The second type of morphological transition of ER and GA is observed in more matured absorptive cells which start appearing lipid particles in Golgi. In this stage, the cis-most cisterna of GA becomes very characteristic mesh structure, which should be expressed as the "net" knitted with tubules rather than lamellar cisterna. Observation of the thick sections stained with zink-iodide-osmium technique reveals large expansion of the net and the peripheries of this net is found to be demarcated by thicker tube which contains small lipid-like particles. The same type of particles are also recognized in the special tubes which connect to the cis-most net. Therefore, these particles are thought to be delivered from apical cytoplasm through the special tube to the cis side of GA.From the several cytochemical results suggest that this tubes appear to possess both ER and Golgi characteristics. These are the main new findings obtained in this project. These results contain very deep implications which would give new insights not only to protein transport from ER to GA,but also to biogenesis of GA. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 野田 亨: "小胞体-その形態と機能の多様性" 電子顕微鏡. 27. 235-244 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 野田 亨: "小胞体とゴルジ装置との移行部の細胞化学的研究" 日本解剖学会第100回総会記念全国学術集会. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noda,T.: "Electron Microscopic Cytochemistry and immunocytochemistry in Biomedicine" CRC Press, 12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Farquhar,M.G.: "Electron Microscopic Cytochemistry and immunocytochemistry in Biomedicine" CRC Press, 39 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 野田 亨: "ライソゾームの基礎とライソゾーム病" 学際企画, 15 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 野田 亨: "電子顕微鏡で見るミクロの世界-生物系-" 学際企画(印刷中), 2

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noda, T.: "Endoplasmic reticulum- its diversity in structures and functions" Electron-microscopy. 27. 235-244 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noda, T.: "Electron Microscopic Cytochemicstry under the high-voltage electron microscope" Electron Microscopic Cytochemistry and immunocytochemistry in Biomedicine (CRC Press). 371-382 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Farquhar, M.G.: "Contributions of Cytochemistry and Immunocytochemistry to our Understanding of the Organization Function of the Golgi Apparatus" Electron Microscopic Cytochemicstry and immunocytochemistry in Biomedicine (CRC Press). 441-479 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noda, T.: "Lysosome and Golgi apparatus" Basic concepts of lysosome and lysosomal disease (Gakusai Kikaku). 41-52 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noda, T.: "Persuit of lysosome by tracer" Basic concepts of lysosome and lysosomal disease (Gakusai Kikaku). 67-69 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 野田 亨: "小胞体とゴルジ装置との移行部の細胞化学的研究" 日本解剖学会第100回総会記念全国学術集会予稿集. 151 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 亨(分担): "ライソゾームの基礎とライソゾーム病、第3章ライソゾームとゴルジ装置" 学際企画, 12 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 亨(分担): "電顕でみるミクロの世界,ゴルジ装置の立体構造" 学際企画(印刷中), 2

    • Related Report
      1994 Annual Research Report
  • [Publications] 野田 亨(分担): "ライソゾームの基礎とライソゾーム病,第3章ライソゾームとゴルジ装置" 学際企画(印刷中),

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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