| Project/Area Number |
05670020
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Oita Medical University |
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Principal Investigator |
SHIMADA Tatsuo Oita Medical University Department of Anatomy Associate Professor, 医学部・解剖学, 助教授 (80080555)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Hirokazu Oita Medical University Department of Anatomy Assitant, 医学部・解剖学, 助手 (70115559)
NAKAMURA Mitsuo Oita Medical University Department of Anatomy Professor, 医学部・解剖学, 教授 (70022770)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | extravascular fluid pathway / Macula cribriformis / pre-lymphocapillary fluid pathway / diaphragm / lymphatic capillary / silver staining / Scanning electron microscopy / Transmission electron microscopy |
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| Research Abstract |
Silver staining, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been used to delineate the structure and function of the extravascular fluid pathway of the diaphragm of monkeys, rats, mice and rabbits.The submesothelial connective tissue was examined by SEM using the NaOHmaceration method.A sieve-like structure, the macula cribriformis, was found in the submesothelial connective tissue on the peritoneal side of both the muscular and tendinous portions.The Macula cribriformis, which consisted of densely-packed collagen fibrils, was oval, spindle and polygonal in shape and ranged in diameter from 20 to 300 um with 5-300 foramina (3-15 um).The macula cribriformis was located between peritoneal mesothelial cells with stomata and subperitoneal lymphatic capillaries.Intraperitoneally-injected latex particles were carried into the diaphragmatic lymphatic capillaries via the peritoneal stomata and the foramina of the macula cribriformis.These results support the hypothesis proposed by Kihara (1956) that the macula cribriformis is a pre-lymphocapillary pathway, one of the extravascular fluid pathways.
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