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Molecular mechanism and ultrastructure of the arterial chemoreceptor organ in chronically hypoxia

Research Project

Project/Area Number 05670024
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionYokohama City University

Principal Investigator

KUSAKABE Tatsumi  Yokohama City Univ.Sch. of Med.Dept.of Anat., Assistant, 医学部, 助手 (80117663)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Tadashi  Yokohama City Univ.Sch. of Med.Dept.of Physiol., Lecture, 医学部, 講師 (60177649)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
KeywordsHypoxia / Carotid body / Ultrastructure / 3-D architecture / Neuropeptides / Immunohistochemistry / Second messenger / Trachea
Research Abstract

The carotid bodies, which are the primary organs for sensing O_2 tension in arterial blood, are enlarged in rat exposed to chronic hypoxia (380 torr, 12 weeks). We noted some ultrastructural features with chronic hypoxia which are characteristic of the amphibian carotid labyrinth glomus cells : 1) incomplete covering of glomus cells with the supporting cell missing over a wide area, 2) long thin cytoplasmic projections in the intervascular stroma, and 3) intimate apposition of the glomus cells and endothelial cells, pericytes, and others. Threedimensional analysis using a combination of an ultra high voltage electron microscope (400kV) and semi-thin sections clearly demonstrated that the incomplete covering of glomus cells is based on a difference in an extent of hypertrophy between glomus cells and supporting cells. Because arterial PO_2 is generally low in amphibia, these ultrastructural similarities may be general features of hypoxic adaptation and facilitate both uptake of oxygen from blood and release of catecholamine into the blood.
When the distribution and abundance of the peptidergic fibers were compared between normoxic and chronically hypoxic carotid bodies and tracheas, there was a difference in number of some peptide-immunoreactive fibers (SP,CGRP,VIP,and others). The increase of these immunoreactive fibers suggests that altered airway reflexes may be a feature of hypoxic adaptation. To clarify a physiological mechanism in the hypoxic adaptation, we are now studying an effect of intracellular second messengers, such as cyclic AMP and others, in axoplasmic transport of nerve fibers in unfixed sections of chronically hypoxic carotid body.

Report

(2 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] T.Kusakabe: "Coexistence of substance p and calcitonin qeue-related peptide in the nerve fibers of the carotid labyrinth of the bullfrog,Rana" Brain Res.603. 153-156 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Immunohistochemical coexistence of calcitonin gene-related peptide and substance P in the nerve fibers of the internal gills of bullfrog(Rana catesbeiana)larvae" Neurosci.Lett. 158. 59-62 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Distribution of FMRFamide-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog,Rana catesbeiana,in corresponding differential interference-contrast(Nomarski)images" Arch.Histol.Cytol.56. 277-282 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Ultrastructure of the glmus cells in the carotid body of chronically hypoxic rats:with special reference to the similarity of amphibian" Anat.Rec.237. 220-227 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kawakami: "Mechanism of inhibitory action of capsaicin on particulate axoplasmic transport in sensory neurons in culture" J.Neurobiol.24. 545-551 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Coexistence of substance P,neuropeptide Y.VIP、and CGRP in the nerve fibers of the carotid labyrinth of the bullfrog,Rana catesbeiana:a double labelling immunofluorescence study in combination with alternate consecutive sections" Cell Tissue Res.276. 91-97 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Localization of immunoreactive neuropeptides in the kidney of the bullfrog,Rana catesbeiana,by immunofluorescence" Cell Tissue Res.278. 41-44 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Distribution of substance P-containing and catecholaminergic nerve fibers in the rabbit carotid body:an immunohistochemical study in combination with catecholamine fluorescent histochemistry" Arch.Histol.Cytol.57. 193-199 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Calcitonin gene-related peptide and substance P in the pharynx and lung of the bullfrog,Rana catesbeiana" Cell Tissve Res.279. 115-121 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Peptidergic innervation in the amphibian carotid labyrinth" Histol.Histopathol.10. 185-202 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kawakami: "Distribution of immunoreactive neuropeptides in the pancreas of the bullfrog,Rana catesbeiana,demonstrated by immunofluorescence" Cell Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Distribution of galanin-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog,Rana catesbeiana:comparison with substance P-immunoreactive nerve fibers" Cell Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.L.Powell: "ARTERIAL CHEMORECEPTORS:Cell to System Effect of chronic hypoxia on rat carotid body and toad carotid labyrinth glomus cell." Ed.by R.G.O'Regan et al., 560 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Coexistence of substance P and calcitonin gene-related peptide in the nerve fibers of the carotid labyrinth of the bullfrong, Rana catesbeiana" Brain Res.603. 153-156 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Immunohistochemical coexistence of calcitonin gene-related peptide and substance P in the nerve fibers of the internal gills of bullfrog (Rana catesbeiana) larvae" Neurosci.Lett.158. 59-62 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Distribution of FMRFamide-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog, Rana catesbeiana, in corresponding differential interference-contrast (Nomarski) images" Arch.Histol.Cytol.56. 277-282 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Ultrastructure of the glomus cells in the carotid body of chronically hypoxic rats : with special reference to the similarity of amphibian glomus cells" Anat.Rec.237. 220-227 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kawakami, et al.: "Mechanism of inhibitory action of capsaicin on particulate axoplasmic transport in sensory neurons in culture" J.Neurobiol.24. 545-551 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Coexistence of substance P,neuropeptide Y,VIP,and CGRP in the nerve fibers of the carotid labyrinth of the bullfrog, Rana catesbeiana : a double labelling immunofluorescence study in combination with alternate consecutive sections" Cell Tissue Res.276. 91-97 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Localization of immunoreactive neuropeptides in the kidney of the bullfrog, Rana catesbeiana, by immunofluorescence" Cell Tissue Res.278. 41-44 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Distribution of substance P-containig and catecholaminergic nerve fibers in the rabbit carotid body : an immunohistochemical study in combination with catecholamine fluorescent histochemistry" Arch.Histol.Cytol.57. 193-199 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Calcitonin gene-related peptide and substance P in the pharynx and lung of the bullfrog, Rana catesbeiana" Cell Tissue Res.279. 115-121 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Peptidergic innervation in the amphibian carotid labyrinth" Histol.Histopathol.10. 185-202 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kawakami, et al.: "Distribution of immunoreactive neuropeptides in the pancreas of the bullfrog, Rana catesbeiana, demonstrated by immunofluorescence" Cell Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe, et al.: "Distribution of galanin-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog, Rana catesbeiana : comparison with substance P-immunoreactive nerve fibers" Cell Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.L.Powell, et al.: "Effect of chronic hypoxia on rat carotid body and toad carotid labyrinth glomus cell" ARTERIAL CHEMORECEPTORS : Cell to system Ed.by R.G.O' Regan et al.365-367 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Kusakabe: "Coexistence of substance P and calcitonin gene-related peptide in the nerve fibers of the carotid labyrinth of the bullfrog,Rana catesbeiana" Brain Res.603. 153-156 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kusakabe: "Immunohistochemical coexistence of calcitonin gene-related peptide and substance P in the nerve fibers of the internal gills of bullfrog (Ranacatesbeiana) larvae" Neurosci.Lett.158. 59-62 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kusakabe: "Distribution of FMRFamide-immunoreactive nerve fibers in the carotid labyrinth of the bullfrog,Rana catesbeiana in corresponding differential interference-contrast (Nomarski)images" Arch.Histol.Cytol.56. 277-282 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kusakabe: "Ultrastructure of the glomus cells in the carotid body of chronically hypoxic rats: with special reference to the similarity of amphibian glomus cells" Anat.Rec.237. 220-227 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kawakami: "Mechanism of inhibitory action of capsaicin on particulate axoplasmic transport in sensory neurons in culture" J.Neurobiol.24. 545-551 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Kusakabe: "Coexistence of substance P,neuropeptide V,VIP,and CGRP in the nerve fibers of the carotid labyrinth of the bullfrog,Rana catesbeiana: a double labelling immunofluorescence study in combination with alternate consecutive sections" Cell Tissue Res.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T:Kusakabe: "Localization of immunoreactive neuropeptides in the kidney of the bullfrog,Rana catesbeiana,by immunofluorescence" Cell Tissue Tes.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.L.Powell: "Chemoreceptors and Chemoreceptor Reflexes in Health and Disease" Effect of chronic hypoxia on rat carotid body and toad carotid labyrinth glomus cell(in press), (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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