| Project/Area Number |
05670026
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Showa University |
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Principal Investigator |
NAKAI Yasumitsu Showa Univ.Sch.of Med., Prof., 医学部, 教授 (60053807)
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| Co-Investigator(Kenkyū-buntansha) |
OU Keihei Showa Univ.Sch.of Med., Assis., 医学部, 助手 (10245821)
OCHIAI Hidehiko Showa Univ.Sch.of Med., Assis.Prof., 医学部, 講師 (40053954)
NAITO Nobuko Showa Univ.Sch.of Med., Associ.Prof., 医学部, 助教授 (30053903)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | hypothalamus / hypophysis / neurosecretory ending / microvesicles / glutamate / glutamate receptor / immunoelectron microscopy / 視床下部下垂体系 / 神経分泌ニューロン / グルタミン酸レセプター / 後葉ホルモン / チロシン水酸化酵素 |
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| Research Abstract |
To clarify the nature and physiological significance of the clear microvesicles within the neurosecretory nerve endings of the rat hypothalamo-hypophysial system, ultrastructural localization of some neuroactive substances in the microvesicles has been examined by immunoelectron microscopy.
1.Glutamate immunoreactivity
Ultrastructural distribution of glutamate immunoreactivity was observed using the post-embedding immunogold staining method. In the neurohypophysis, glutamate immunoreactivity was found to be localized in the area where numerous microvesicles aggregate Many immunogold particles were observed in the interior of microvesicles.
2.Glutamate receptor immunoreactivity
Immunoparticles for N-methyl-D-Aspartate (NMDA)-RI receptor was localized to the microvesicles within the nerve endings in the periventricular uncles of the hypothalamus.
3.Tyrosine hydroxylase (TH) immunoreactivity
TH-like immunoreactivity was found in the aggregate area of microvesicles within the catecholamine-containing nerve endings of the hypothalamus (A12, A14).
4.Synaptophysin immunoreactivity was found in the microvesicles within the neurosecretory endings of the neurohypophysis. The above findings of ultrastructural localizations of glutamate and its receptor suggest that glutamate play some important roles of hormone secretion in the neurosecretory endings through autorine mechanism.
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