| Project/Area Number |
05670031
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | Tokyo Women's Medical College |
|---|
Principal Investigator |
AIKAWA Eizo Tokyo Women's Medical College, Medicine, Professor, 医学部, 教授 (40048989)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YASUI Hiroshi Women's Medical College, Medicine, Assistant, 医学部, 助手 (60210241)
NAKAZAWA Tomoko Women's Medical College, Medicine, Assistant, 医学部, 助手 (40075437)
KANDA Naotoshi Tokyo university of Agriculture & Technology, Veterinaty Science, Professor, 農学部, 教授 (40075429)
NISHIKAWA Megumi Women's Medical College, Medicine, Lecturer, 医学部, 講師 (80120020)
片桐 展子 東京女子医科大学, 医学部, 講師 (30075428)
|
|---|
| Project Period (FY) |
1993 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | steroid sulfatase / arylsulfatase C / in situ hybridization / enzyme histochemistry / molecular biology / immuno histochemistry / 免役組織化学 / Steroid sulfatase |
|---|
| Research Abstract |
The distribution of steroid sulfatase (STS) activity and mRNA in human uroginital tissues was analyzed by enzyme histochemistry and in situ hybridization. STS activity demonstrated by the presence of 4-methylumbelliferyl sulfate was clearly localized in proximal tubular cells. The reaction products were located at the endoplasmic reticulum. In situ hybridization using a STS cDNA probe with digoxigenin-5 nm gold particles demonstrated STS mRNA to be located primarily in the cortical-medullary junction. It was not detected in either the mesangial or endothelial cells of the glomeruli. The ultrastructural localization of STS mRNA was mainly visualized between the mitochondria in the proximal tubules. These results indicate that STS is synthesized in the proximal tubules and glomerular epithelial cells of the human kidney. Moreover, STS may be related to the metabolism of steroid hormones in these segments of nephrons.
|
