| Project/Area Number |
05670034
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | NAKAMURA GAKUEN UNIVERSITY,FACULTY OF HOME ECONOMICS |
|---|
Principal Investigator |
FUJITA Mamoru Nakamura Gakuen University, Faculty of Home Economics Professor, 家政学部, 教授 (60037471)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
BABA Ryoko Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 副手
MATSUKUMA Miki Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 助手 (40259669)
HARA Takayuki Nakamura Gakuen University, Faculty of Home Economics Assistant professor, 家政学部, 助教授 (10164998)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Keywords | HDL / ultrastructure / cytochemistry / histochemistry / LDL / liver / hepatocyte |
|---|
| Research Abstract |
We used histochemistry, immunohistochemistry, electromicroscopy, cytochemistry and biochemistry to investigate the endocytic pathway of high density lipoprotein (HDL) in several cells. In addition, we examined the endocytic pathway of low density lipoprotein (LDL) and horseradish peroxidase (HRP) in several cells.
HDL and LDL were isolated from normal rat and rabbit blood by sequential ultracentrifugation. Rat HDL,rat LDL,rabbit HDL and rabbit LDL were labeled with biotin.
The initial interaction of HDL-biotin and LDL-biotin with hepatocytes occurred at binding sites distributed over the basolateral (sinusoidal) plasma membrane. Ligand internalization was involved coated puits, coated vesicles, early endosomes, late endosomes, and lysosomes. However, the binding sites distributed over the endocytic membrane were difference between HDL-biotin and LDL-biotin.
Internalization of HDL-biotin in hepatocytes and adrenal cortical cells of rabbit was no inhibition by a hypercholesterol diet containing 0.5% cholesterol for 4 months.
|
