| Project/Area Number |
05670089
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
HIGUCHI Hiroshi Osaka University School of Medicine, Department of Pharmacology I,Associate professor, 医学部, 助教授 (30150337)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Neuropeptide Y / enkepalin A / gene expression / transcription / transcriptiona factor / cis-element / neural differentiation / depolarization / cis-element / depdarization / 降圧剤 / SHR / pheochromocytoma / CAT assay / transcriptional factor |
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| Research Abstract |
The aim of this project is to elucidate the transcriptional regulation of neuron-specific genes by drugs and identification of transcriptional factors which are involved in the regulation. For this purpose, we used the cloned neuropeptide Y and enkephalin A genes by molecular biologial techniques. The results are as follows ;
(1) The positive regulation of NPY gene by membrane depolarization occurs at the transcriptional level.
(2) Three enhancer elements such as NGF responsive element, depolarization responsive element, and glucocorticoide responsive element are identified in the promoter regon of NPY gene.
(3) Several DNA-binding factors are found to bind to NGF responsive element.
(4) One of these factors, NDF1 was increased in binding activity with NGF treatment.
(5) The future goal is to clarify whether these transcriptional factors are important for gene regulation by drugs and neural differentiation, and the coning study is now going on.
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