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Structure and expression of the human 2-oxoglutarate dehydrogenase gene

Research Project

Project/Area Number 05670149
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionNagasaki University

Principal Investigator

KOIKE Kichiko (1994)  Nagasaki University Sch.of Med.Associ.Prof., 医学部, 助教授 (80039619)

小池 吉子 (1993)  長崎大学, 医学部, 助教授 (10039521)

Co-Investigator(Kenkyū-buntansha) MATSUO Sachiko  Nagasaki University, Inst.Trop.Med.Instructor, 熱帯医学研究所, 助手 (80039916)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords2-oxoglutarate dehydrogenase / gene / exon / intron / coenzyme TPP / transcription start point / chromosomal localization / ヒト / クローニング / 塩基配列 / 発現調節
Research Abstract

The 2-oxoglutarate dehydrogenase (OGDH) complex that catalyzes oxidative decarboxylation of 2-oxoglutarate. It is apparent that the OGDH complex is composed of three different component enzymes. The OGDH,one of the component enzymes of the complex catalyzes the TPP-dependent irreversible decarboxylation and the subsequent reductive succinylation of the lipoyl moiety. We recentry cloned the human cDNA encoding OGDH from fetal liver cDNA library. To obtain further insights into the regulation of its expression, we tried to isolate genomic OGDH clones using the human cDNA as a probe and characterize the OGDH gene structure.
Genomic clones covering the entire sequence of the gene encoding human OGDH were isolated by screening leukocyte and placenta genomic libraries with radio- and digoxigenin-labeled human OGDH cDNAs. The human OGDH gene contains 22 exons spanning approximately 85 kb. All exon/intron splice junctions follow the GT/AG rule. The results of primer extension analysis imply that the OGDH gene transcription start point is a thymine base 55 bp upstream of the ATG start codon. The 5'-flanking region of the OGDH gene lacked canonical TATA or CAAT boxes. Using DNAs from human * rodent somatic cell hybrids that segregate human chromosome in conjunction with fluorescence in situ hybridization, human OGDH gene was assigned to chromosome arm 7p, an area that corresponds to the boundary between bands 7p13 and 7p14.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(17 results)

All Other

All Publications (17 results)

  • [Publications] 小池 吉子: "アルツハイマー病脳・線維芽細胞の2-オキソグルタル酸脱水素酵素異常" 神経研究の進歩. 37. 1023-1030 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kwan Fu Rez Sheu: "Abnormality of -ketoglutarate dehybrogenase complex in fibroblasts from familial Alzheimer′sdisease" Annals of Neurology. 35. 312-318 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kichiko Koike: "The gene encoding human 2-oxoglutarate dehydro-genase:structural organizatior and mappirg to chrosome 7p13-p14" Gene. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kichiko Koike: "Flavins and Flavoproteins" Walter de Gruyte & Co., 889 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kichiko Koike: "Abnormality of the 2-oxoglutarate dehydrogenase in brains and fibroblasts from familial Alzheimer's disease" Adv.Neurol.Sci.37. 1023-1030 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kwan-Fu Rex Sheu: "Abnormality of the alpha-ketoglutarate dehydrogenase complex in fibroblasts from familial Alzheimer's diseas" Annals of Neurology. 35. 312-318 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kichiko Koike: "Eukaryotic lipoamide dehydrogenase molecular genetic and structural aspects" Flavins and Flavo-proteins, Walter de Gruyter & Co.509-517 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kichiko Koike: "The gene encoding human 2-oxoglutarate dehydrogenase : structural organization and mapping to chromosome 7p13-p14" Gene. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小池 吉子: "アルツハイマー病脳・線維芽細胞の2-オキソグルタル酸脱水素酵素異常" 神経研究の進歩. 37. 1023-1030 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kwan-Fu Rex Sheu: "Abnormality of the α-ketoglutarate dehydrogenase complex in fibroblasts from familial Alzheimer´s disease" Annals of Neurology. 35. 312-318 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kichiko Koike: "The gene encoding human 2-oxoglutarate dehydrogenase: structural organization and mapping to chromosome 7p13-p14" Gene. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kichiko Koike: "Flavins and Flavoproteins" Walter de Gruyter & Co., 889 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kichiko Koike: "Cloning and nucleotide sequence of cDNA encoding human 2-oxoglutarate dehydrogenase(lipoamide)" Proc.Natl.Acad.Sci.USA. 89. 1963-1967 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小池吉子: "ヒトピルビン酸脱水素酵素alpha、betaサブユニットの遺伝子構造" ビタミン. 67. 455-467 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小池吉子: "2-オキソグルタル酸脱水素酵素遺伝子の単離および構造解析" 生化学. 65. 758 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小池吉子: "アルツハイマー病脳・線維芽細胞の2-オキソグルタル酸脱水素酵素異常" 神経研究の進歩. 37. 1023-1030 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kwan-Fu Rex Sheu: "Abnormality of the alpha-ketoglutarate dehydrogenase complex in fibroblasts from familial Alzheimer′s disease" Annals of Neurology. (in press).

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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