Molecular and biological characterization of fusion regulatory protein (FRPs) : anti-FRP mAbs induced virus-mediated cell fusion via an integrin system
| Project/Area Number |
05670153
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Science University of Tokyo |
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Principal Investigator |
OHTA Hisataka Science Univ.of Tokyo, Faculty of Science, assistant, 理学部, 助手 (40223838)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Yasuhiko Mie Univ., School of Medicine, Professor, 医学部, 教授 (00022872)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | HIV / Integrin / Fusion-regulation / integrin / fusion regulation |
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| Research Abstract |
We have recently isolated monoclonal antibodies (mAbs) which enhance cell fusion in NDV-infected human cells. These molecules were designated as fusion regulatory protein (FRP-1,2) and thier propaties were well characterized using immunoblotting, immunoprecipitaion, kinetic analysis and indirect immunostaining of varioius cell lincs. Objetive of this research are : (1) Quantitative preparation of FRP-1 (2) Amino acid sequence analysis of FRP-1 (3) Molecular cloning of FRP-1 (4) Cellular mechanism of FRP-1. From 1994, Amino acid analysis suggested that FRP-1 has high homology to 4F2 antigen (CD98). Monovlonal Ab to CD98 induce virus infected cell fusion. CD98 expressed cell was cross reacted to mAb of FRP-1. We concluded that FRP-1 is identical to CD98. Moreover fusion regulatory protein related molecules were idcntifed as vimentin and desmin by immunoscreening of lgt ll library.
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Report
(3 results)
Research Products
(7 results)
