| Project/Area Number |
05670155
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
MITA Takashi Sch.of Medicine, University of Occupational and Environmental Health Professor, 医学部, 教授 (20076988)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Osamu Sch.of Medicine Assistant, 医学部, 助手 (70248558)
FUETA Yukiko Sch.of Medical Tech.Assistant, 助手 (10132482)
HIRANO Hideyasu Sch.of Medicine Assis.Prof., 医学部, 講師 (50040241)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | epilepsy gene / El mouse / RFLP / cDNA library / synaptosome / Isocitrate dehydrogenase / mutation / S-Adenosylhomocysteine hydrolase / 突然変異 / Northern blotting / てんかん / 2次元,ゲル電気泳動 / シナプトソーム / 歯状回 / GABA |
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| Research Abstract |
Two genes associated with epilepsy of El mice were isolated, one of which encodes S-Adenosylhomocysteine hydrolase (S-AdoHocysHlase). It was derived from cDNA library of liver poly A^+ RNAs of 1 day postnatal El mice. A clone named pELD1 which contains the above gene, revealed Pst I-RFLP on the genomic DNAs between group A (ddY,BALB/c, C3H/He) and group B (El, C57BL/6N,DBA/1J). The latter group includes epileptic seizure predisposed strains. This means that S-AdoHocysHlase gene is associated with the epileptogenesis. Another one was identified by amino acid sequencing to be NADP specific isocitrate dehydrogenase gene, which encodes a 45kDa brain synaptosomal protein appeared in 2D-SDS-PAGE,giving 0.2isoelectric point difference between El and ddY mice. The structure of genes in detail are now under investigation.
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