| Project/Area Number |
05670163
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MACHINAMI Rikuo Professor, Faculty of Medicine, Univ, of Tokyo, 医学部(医), 教授 (30010052)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Masahiko Assistant, Faculty of Medicine, Univ, of Tokyo, 医学部, 助手 (80251304)
MORI Masaya Assistant, Faculty of Medicine, Univ, of Tokyo, 医学部, 助手 (90210137)
HORIUCHI Hajime Assistant, Faculty of Medicine, Univ, of Tokyo, 医学部, 助手 (10190240)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Bone and soft tissue tumor / Histological grading / ATPase / Cytogenetics / Tumor associated genes / Immunohistochemistry / Molecular biology / Flow cytometry |
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| Research Abstract |
Monoclonal antibody for calcium ATPase from the muscle produced by 2 of us (M.K.& H.H.) was used for immunohistochemical study of bone and soft tissue tumors. Rhabdomyosarcoma, synovial sarcoma, chordoma and chondrosarcoma were positive for calcium ATPase. It is interestion that sarcomas with epithelial differentiation are positive for ATPase.
A case of extraskeletal myxoid chondrosarcoma was examined cytogenetically. Mode of the chromosome was 46. Translocation t (9 ; 22) was found in 22 of 23 cells examined. Abnormalities of chromosome 9 and 22 seem to be specific for extraskeletal myxoid chondrosarcoma.
It has been reported that chromosomal abnormalities of t (12 ; 16) and associated chimera gene TLS-CHOP are specific for myxoid liposarcoma. TLS-CHOP chimera gene was examined for 8 cases of myxoid liposarcoma by RT-PCR.Seven of 8 cases were positive for this chimera gene, while one case of pleomorphic liposarcoma and 3 cases of myxoid malignant fibrous histiocytoma were negative.
Sixteen cases of chondrosarcoma were examined for p53 gene and associated protein by mplecular biology and immunohistochemistry. Two of 16 cases showed positive staining for p53 protein and point mutation of p53. These 2 chondrosarcomas were high grade (Grade 3) histologically.
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