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Expression of neuron-specific B-tubulin protein in non-neoplastic and neoplastic tissues.

Research Project

Project/Area Number 05670179
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionKitasato Univ.School of Med.

Principal Investigator

KAMEYA Toru  Kitasato Univ.Sch.of Medicine Professor, 医学部, 教授 (50101035)

Co-Investigator(Kenkyū-buntansha) NARITAKA Heiji  Keio Univ. Sch. Medicine Assistant Professor, 医学部, 助手 (60218063)
渡辺 純  北里大学, 医学部, 助手 (10201188)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
KeywordsNeuron-specific beta-tubulin isotype / Neuroendocrine tumor / Ultimobranchial glands / Carotid body / Immunohistochemistry / 鰓後体 / ニューロン特異ClassIIIβ-tubulin / アミン・ペプチドホルモン産生腫瘍 / 頸動脈体 / 末梢神経 / Class IIIbeta-tubulin isotype / Pituifary adenoma / ultimobranchiel body / Neuronal marker
Research Abstract

The incidince of expression of neuron-specific ClassIIIbeta isotype tubulin was examined by use of a monclonal antibody TuJ1 in several types of amine-peptide producing endocrine tumors. Forty-three out of 61 pituitary adenomas (73%) expressed this isotype. This indicated an ectopic expression of the tubulin isotype. 100% of adrenal pheochromocytomas, 45% of medullary thyroid carcinomas, 30% of pancreatic endocrine tumors, 25% of carcinoid tumors, and 10% of pulmonary small cell carcinomas expressed the tubulin isotype.
The localization of the neuron-specific beta-tubulin isotype in the ultimobranchial glands from chickens at various stages of development was examined by immunostaining, using the same antibody TuJ1. Many C cells were intensely stained for the isotype at 8 days incubation. The staining started to decrease at late stages of embryonic development. After hatching, the staining became weaker and, in 10-day-old chickens, restricted to the nerve fibers of the ultimobranchial gland.
Development of the carotid body and the glomus cell groups in the wall of the common carotid artery was examined in chickens at various developmental stages by immunohistochemistry using the same antibody TuJ1. From 9 days of incubation, the immunoreactive cells continuing with the distal vagal ganglion began to enter into the carotid body anlage. At 12 days of incubation, a large portion of the body were occupied by the immunoreactive cells The present study demonstrates that the glomus cells in the carotid body are emigtres that arrive in each residential place from the distal vagal ganglion.
The isotype was localized at neurotubules of non-neoplastic peripheral nerves but not localized in other sites of neuronal cells by electron microscopic immunocytochemistry.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Furuhata,S.: "Immunohistochemical analysis of 61 pituitary adenomas with a monoclonal antibody to the neuron-specific β-tubulin isotype." Acta Neuropathol. 86. 518-520 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kameda,Y.: "Immunohistochemical localization of a neuron-specific β-tubulin isotype in the developing chicken ultimobranchial glands" Brain Research. 628. 121-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kameda,Y.: "Glomus cell differentation in the carotid body region of chick embryas studied by neuron-specific ClassIIIβ-tubulin isotype and Leu-7 monoclonal antibodies." Journal Comparative Neurology. 348. 531-543 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yagishita,S.: "Immunohistochemical and ultrastructurial study of cerebellar medulloblastoma-neuroblastona group and cerebral primitive neuroectodermal tumor" Neuropathology. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furuhata, S., Kameya, T., etal: "Immunohistochemical analysis of 61 pituitary adenomas with a monoclonal antibody to the neuron-specific beta-tubulin isotype" Acta Neuropathol. 86. 518-520 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kameda, Y., Kameya, T., etal: "Immunohistochemical localization of a neuron-specific beta-tubulin isotype in the developing chicken ultimobranchial glands" Brain Research. 628. 121-127 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kameda, Y., Kameya, T., etal: "Glamus cell differentiation in the corotid body region of chick-embryos studied by neuron-specific Class III beta-tubulin isotype and Leu-7 monoclonal antibodies" J.Conparative Neurology. 348. 531-543 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yagishita, S., Kameya, T., etal: "Immunohistochemical and Ultrastructurol study of cerebellar modullo blastomaneuroblastom groups and cerebral primitive neuroectodermal tumor" Neuropathology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kameda,Y.: "Glomus cell differentiation in the carotid body region of chick embryos studied by neuron-specific ClassIIIβ-tubulin isotype and leu-7 monoclonal antibodies." Journal of Comparative Neurology. 348. 531-543 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yagishita,S.: "Immunohistochemical and ultrastructural study of cerebellar medullcblastoma group and cerebral primitive neuroctodermal tumor." Neuropathology. (発表予定).

    • Related Report
      1994 Annual Research Report
  • [Publications] Shigeru Furuhata: "Immunohistochemical analysis of 61 pituitary adenomas with a monoclonal antibody to the neuron-specific beta-tubulin isotype" Acta Neuropathologica. 86. 518-520 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoko Kameda: "Immunohistochemical localization of a neuron-specific beta-tubulin isotype in the developing chicken ultimobranchial glands" Brain Research. 628. 121-127 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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